Antigenic analysis of the influenza B virus hemagglutinin protein  

作　　者：Mengyi Zhang Chaoying Yang Xi Wu Yifei Wang Lijie Wang Qianqian Cui Jincheng Tong Yimeng An Meina Cai Shishi Cheng Qi Jiang Yulin Wang Chenyan Zhao Youchun Wang Weijin Huang 

机构地区：[1]Division of HIV/AIDS and Sex-Transmitted Virus Vaccines,Institute for Biological Product Control,National Institutes for Food and Drug Control(NIFDC),Beijing,102629,China [2]National Institutes for Food and Drug Control,Chinese Academy of Medical Science&Peking Union Medical College,Beijing,100730,China [3]National Vaccine&Serum Institute,Beijing,101111,China [4]Institute of Medical Biology,Chinese Academy of Medical Science&Peking Union Medical College,Kunming,650031,China

出　　处：《Virologica Sinica》2025年第1期80-91,共12页中国病毒学(英文版)

基　　金：supported by a grant from the National Key R&D Program of China(No.2021YFC2301700);the Ministry of Science and Technology(China)of China(No.2021YFC2302500);the Establishment of a method for detecting neutralizing antibodies with pseudotyped viruses of China Pharmacopeia[grant number 2021S03].

摘　　要：Influenza B viruses(IBVs)primarily infect humans and are a common cause of respiratory infections in humans.Here,to systematically analyze the antigenicity of the IBVs Hemagglutinin(HA)protein,31 B/Victoria and 19 B/Yamagata representative circulating strains were selected from Global Initiative of Sharing All Influenza Data(GISAID),and pseudotyped viruses were constructed with the vesicular stomatitis virus system.Guinea pigs were immunized with three doses of vaccines(one dose of DNA vaccines following two doses of pseudotyped virus vaccines)of the seven IBV vaccine strains,and neutralizing antibodies against the pseudotyped viruses were tested.By comparing differences between various vaccine strains,we constructed several pseudotyped viruses that contained various mutations based on vaccine strainBV-21.The vaccine strains showed good neutralization levels against the epidemic virus strains of the same year,with neutralization titers ranging from 370 to 840,while the level of neutralization against viruses prevalent in previous years decreased 1–10-fold.Each of the high-frequency epidemic strains of B/Victoria andB/Yamagata not only induced high neutralizing titers,but also had broadly neutralizing effects against virus strains of different years,with neutralizing titers ranging from1000 to 7200.R141G,D197 N,and R203K were identified as affecting the antigenicity of IBV.These mutation sites provide valuable references for the selection and design of a universal IBV vaccine strain in the future.

关 键 词：Influenza B virus(IBV) ANTIGENICITY Vaccine strains Pseudotyped viruses 

分 类 号：R392[医药卫生—免疫学]