TNFAIP3对胶质母细胞瘤细胞增殖、侵袭和迁移的影响及相关机制  

Effect of TNFAIP3 on the proliferation,invasion and migration of glioblastoma cells and related mechanisms

作　　者：岳成龙 韩正中 朱炳鑫 朱永琦 李政委 YUE Chenglong;HAN Zhengzhong;ZHU Bingxin;ZHU Yongqi;LI Zhengwei(Department of Neurosurgery,Xuzhou Children′s Hospital Affiliated to Xuzhou Medical University,Xuzhou,Jiangsu 221002,China)

机构地区：[1]徐州医科大学附属徐州儿童医院神经外科,江苏徐州221002

出　　处：《徐州医科大学学报》2025年第3期228-234,共7页Journal of Xuzhou Medical University

基　　金：徐州市卫生健康委青年项目(XWKYHT20230005);徐州市科技局基础研究计划面上项目(KC21050)。

摘　　要：目的探讨肿瘤坏死因子α诱导蛋白3(TNFαinduced protein 3,TNFAIP3)在胶质母细胞瘤(GBM)细胞增殖、侵袭和迁移中的作用及其相关机制。方法利用GEPIA数据库分析TNFAIP3 mRNA在GBM中的表达水平,并分析其对患者生存的影响。通过CCK-8实验、集落形成实验以及细胞侵袭和迁移实验,评估沉默或过表达TNFAIP3对GBM细胞增殖、侵袭和迁移的影响。此外,采用生物信息学分析及蛋白质印迹法进一步探讨TNFAIP3调控GBM细胞增殖、侵袭和迁移的机制。结果TNFAIP3 mRNA在GBM中呈高表达,并与患者预后不良相关。沉默TNFAIP3可抑制GBM细胞的增殖、侵袭和迁移能力,而过表达TNFAIP3则促进GBM细胞的增殖、侵袭和迁移能力。TNFAIP3可能通过激活JAK酪氨酸蛋白激酶2(JAK2)-信号传导和转录激活因子3(STAT3)信号通路,促进GBM细胞的增殖、侵袭和迁移。结论TNFAIP3在GBM中高表达,并通过激活JAK2-STAT3信号通路,促进GBM的细胞增殖、侵袭和迁移。Objective To investigate the role of tumor necrosis factorαinduced protein 3(TNFAIP3)in the proliferation,invasion,and migration of glioblastoma(GBM)cells and its underlying mechanisms.Methods The GEPIA database was used to analyze the mRNA levels of TNFAIP3 in GBM and its impact on patient survival.The effect of silencing or overexpressing TNFAIP3 on the proliferation,invasion,and migration of GBM cells were assessed by CCK-8 assay,colony formation assay,and cell invasion and migration assays.Furthermore,bioinformatics analysis and Western blot were employed to explore the mechanisms by which TNFAIP3 regulates the proliferation,invasion,and migration of GBM cells.Results TNFAIP3 mRNA was significantly up-regulated in GBM and associated with poor prognosis.Silencing TNFAIP3 inhibited the proliferation,invasion,and migration adilities of GBM cells,while overexpressing TNFAIP3 enhanced these abilities.Further analysis indicated that TNFAIP3 might promote the proliferation,invasion,and migration GBM cells by activating the JAK tyrosine protein kinase 2(JAK2)-signal transduction and transcription activator 3(STAT3)signaling pathway.Conclusions TNFAIP3 is highly expressed in GBM and promotes the proliferation,invasion,and migration of GBM cells through the activation of the JAK2-STAT3 signaling pathway.

关 键 词：胶质母细胞瘤 肿瘤坏死因子α诱导蛋白3 JAK酪氨酸蛋白激酶2 信号传导和转录激活因子3 细胞增殖 细胞侵袭 细胞迁移 

分 类 号：R739.41[医药卫生—肿瘤]