lncRNA DLG 1-AS 1靶向miR-103 a-3 p对肺癌细胞A549增殖、迁移和侵袭的影响  

Effect of LncRNA DLG 1-AS 1 on Proliferation,Migration and Invasion of Lung Cancer Cell A549 by Targeting miR-103 a-3 p

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作  者:张春玲 李治岐 左燕雨 ZHANG Chunling;LI Zhiqi;ZUO Yanyu(Department of Pulmonary Oncology,Cancer Hospital of Nanyang Central Hospital,Nanyang 473000,China)

机构地区:[1]南阳市中心医院肿瘤医院肺部肿瘤内科,南阳473000

出  处:《福建医科大学学报》2025年第1期1-8,共8页Journal of Fujian Medical University

基  金:河南省医学科技攻关计划联合共建项目(LHGJ20200907)。

摘  要:目的探讨长链非编码RNA(lncRNA)DLG 1反义RNA1(DLG 1-AS 1)是否通过靶向miR-103 a-3 p影响肺癌细胞A549的增殖、迁移和侵袭。方法qRT-PCR分析DLG 1-AS 1和miR-103 a-3 p在肺癌组织中的表达量;双荧光素酶报告基因检测和RNA免疫沉淀(RIP)技术确定DLG 1-AS 1与miR-103 a-3 p的靶向关系;qRT-PCR检测干扰DLG 1-AS 1表达后A549细胞的miR-103 a-3 p表达量;CCK-8实验和克隆形成实验分析DLG 1-AS 1和miR-103 a-3 p表达对A549细胞增殖的影响;Transwell实验分析DLG 1-AS 1和miR-103 a-3 p表达对A549细胞迁移和侵袭的影响;Western-blot法检测上皮细胞钙黏蛋白(E-cadherin)和神经钙黏蛋白(N-cadherin)的表达情况。结果与癌旁组织比较,肺癌组织的DLG 1-AS 1表达量显著升高(P<0.05),而miR-103 a-3 p表达量显著下降(P<0.05)。miR-103 a-3 p是DLG 1-AS 1的靶基因,干扰DLG 1-AS 1表达后,miR-103 a-3 p表达量显著升高(P<0.05),A549细胞活力、克隆形成数、迁移和侵袭细胞数、N-cadherin表达量均显著下降(P<0.05),E-cadherin表达量显著上升(P<0.05)。与干扰DLG 1-AS 1表达比较,同时干扰DLG 1-AS 1和miR-103 a-3 p表达后,A549细胞活力、克隆形成数、迁移和侵袭细胞数、N-cadherin表达量均显著上升(P<0.05),而E-cadherin表达量显著下降(P<0.05)。结论干扰DLG 1-AS 1可上调miR-103 a-3 p表达,进而降低肺癌细胞A549的增殖、迁移和侵袭能力。Objective To investigate whether the long non-coding RNA(lncRNA)DLG 1 antisense RNA1(DLG 1-AS 1)affects the proliferation,migration and invasion in lung cancer cell A549 by targeting miR-103 a-3 p.Methods The expression levels of DLG 1-AS 1 and miR-103 a-3 p in lung cancer tissues was analyzed by qRT-PCR.The dual luciferase reporter assay and RNA immunoprecipitation(RIP)assay was used to determine the targeting relationship between DLG 1-AS 1 and miR-103 a-3 p,and qRT-PCR was used to detect the expression of miR-103 a-3 p in A549 cells after interfering with DLG 1-AS 1.CCK-8 experiment and colony formation experiment was used to analyze the effect of DLG 1-AS 1 and miR-103 a-3 p expression on the proliferation of A549 cells.Transwell experiment was used to analyze the effects of DLG 1-AS 1 and miR-103 a-3 p expression on the migration and invasion of A549 cells.Western-blot was used to measure the expression levels of epithelial cell calreticulin(E-cadherin)and neurocadherin(N-cadherin).Results Compared with the paracancerous tissues,the expression of DLG 1-AS 1 in lung cancer tissues was significantly increased(P<0.05),whereas the expression of miR-103 a-3 p was significantly reduced(P<0.05).miR-103 a-3 p is the target gene of DLG 1-AS 1,and after interference with DLG 1-AS 1 expression,the expression of miR-103 a-3 p was significantly increased(P<0.05).After interference with DLG 1-AS 1 expression,A549 cell viability,number of colony formation,number of migration and invasion cells,and expression of N-cadherin protein were remarkably reduced(P<0.05),while expression of E-cadherin protein were remarkably increased(P<0.05).Compared with the interference of DLG 1-AS 1 expression,A549 cell viability,number of colony formation numbers,number of migration and invasion cells,and expression of N-cadherin protein were remarkably increased after interfering with both DLG 1-AS 1 and miR-103 a-3 p expression(P<0.05),while expression of E-cadherin protein was significantly reduced(P<0.05).Conclusion Interference with DLG1-A

关 键 词:DLG 1-AS 1 miR-103 a-3 p 肺癌 细胞增殖 迁移 侵袭 

分 类 号:R734.2[医药卫生—肿瘤]

 

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