癌基因c-MYC表达与线粒体代谢酶DLAT/DLST及胰腺导管腺癌进展相关  

Correlation of oncogene c-MYC expression with mitochondrial metabolic enzyme DLAT/DLST and progression of pancreatic ductal adenocarcinoma

作　　者：徐也婷 秦子亿 王钰铖 吴焕文[2] 鞠瑞[1] 郭磊[1] XU Yeting;QIN Ziyi;WANG Yucheng;WU huanwen;JU Rui;GUO Lei(Department of Pharmacology,Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC,Beijing 100005;Department of Pathology,Peking Union Medical College Hospital,CAMS&PUMC,Beijing 100730,China)

机构地区：[1]中国医学科学院基础医学研究所,北京协和医学院基础学院药理系,北京100005 [2]中国医学科学院,北京协和医学院,北京协和医院病理科,北京100730

出　　处：《基础医学与临床》2025年第4期450-455,共6页Basic and Clinical Medicine

基　　金：呼吸和共病全国重点实验室开放课题基金(2060204);科技创新2030-“脑科学与类脑研究”重大项目2021年度定向委托项目(#2021ZD0201100);“人脑组织资源库及地区脑库协作网络平台”课题1(#2021ZD0201101)。

摘　　要：目的探究胰腺癌患者恶性导管上皮细胞中c-MYC表达与线粒体代谢之间的相关性。方法利用GEPIA数据库分析c-MYC表达和患者总生存期的相关性;通过免疫组化染色检测患者肿瘤组织中c-MYC的表达情况;在GEPIA数据库中比较肿瘤组织和正常组织DLAT和DLST的基因表达差异;利用HPA数据库分析患者肿瘤组织中c-MYC与DLAT、DLST表达水平之间的相关性;通过免疫荧光染色评价患者肿瘤组织中DLAT和DLST的表达水平。结果c-MYC基因高表达与患者总生存期呈显著负相关(P<0.01),c-MYC蛋白水平与PanIN病理分级呈正相关;与正常组织相比,胰腺癌细胞中DLAT和DLST基因表达水平增加(P<0.01);c-MYC的蛋白水平与DLAT和DLST蛋白水平呈显著正相关(P<0.01,P<0.001)。结论胰腺导管腺癌细胞中线粒体代谢酶DLAT、DLST高表达与c-MYC表达水平显著相关,c-MYC表达水平随胰腺癌进展增加。Objective To investigate the correlation between c-MYC expression and mitochondrial metabolism in malignant duct epithelial cells of pancreatic cancer patients.Methods GEPIA database was used to analyze the correlation between c-MYC expression and overall survival.The expression of c-MYC in tumor tissues was detected by immunohistochemical staining.The difference of DLAT and DLST gene expression between tumor and normal tissues was compared in GEPIA database.HPA database was used to analyze the correlation between c-MYC and DLAT,DLST expression in tumor tissues.The expression level of DLAT and DLST in tumor tissues was evaluated by immunofluorescence staining.Results The high expression of c-MYC gene was negatively correlated with overall survival(P<0.01).The level of c-MYC protein was positively correlated with the pathological grade of PanIN.Compared with normal tissues,the expression of DLAT and DLST genes in pancreatic cancer cells was increased(P<0.01).The protein level of c-MYC was positively correlated with those of DLAT and DLST(P<0.01,P<0.001).Conclusions The high expression of mitochondrial metabolic enzymes DLAT and DLST in pancreatic ductal adenocarcinoma cells is significantly correlated with the expression level of c-MYC,which increases with the progression of pancreatic cancer.

关 键 词：胰腺癌 C-MYC 线粒体代谢 

分 类 号：R735.9[医药卫生—肿瘤]