精异丙甲草胺及助剂对作物根际微生物的影响  

Effects of S-metolachlor and its auxiliary on the rhizosphere microorganisms of crop

作　　者：杨超铭 于鑫 成宏二 米长虹 王伟 赵倩 YANG Chaoming;YU Xin;CHENG Honger;MI Changhong;WANG Wei;ZHAO Qian(Agro-Environmental Protection Institute,Ministry of Agriculture and Rural Affairs,Tianjin 300191,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China;Forensic Center of Agro-Ecological Environment and Agro-Product Quality Safety,Tianjin 300191,China)

机构地区：[1]农业农村部环境保护科研监测所,天津300191 [2]中国农业科学院研究生院,北京100081 [3]农业生态环境及农产品质量安全司法鉴定中心,天津300191

出　　处：《农业环境科学学报》2025年第3期794-805,共12页Journal of Agro-Environment Science

基　　金：中国农业科学院创新工程项目(CAAS-CSGLCA-202302);天津市自然科学基金重点项目(23JCZDJC00480)。

摘　　要：为探讨精异丙甲草胺(S-ME)及其助剂对红小豆根际微生物群落的影响,选取农业生产中所允许的S-ME施用浓度50mL·hm^(-2)及典型有机硅助剂浓度0.25%和0.5%,通过16S rRNA扩增子测序技术分析了红小豆根际细菌群落对S-ME及其助剂的响应。结果表明:单独施用S-ME会导致微生物多样性降低,尤其是有益微生物如酸杆菌属(RB41)的丰度显著下降;而与助剂配施可缓解这种抑制效应,促进鞘氨醇单胞菌(Sphingomonas)和盖氏菌(Gaiella)等微生物的生长。此外,0.5%有机硅助剂处理显著增加了芽殖球菌(Blastococcus)和寡养单胞菌(Stenotrophomonas)的丰度,提高了微生物群落多样性,共现网络关系也反映了助剂的协同促进作用。研究发现,助剂通过调节根际微环境和促进有益微生物生长,可有效缓解除草剂对根际微生物的毒害效应。The aim of this study was to investigate the effects of S-metolachlor(S-ME)and its additives on the inter-root microbial community of red bean(Vigna angularis).In this study,six treatments were designed,including the application of the chloroacetanilide herbicide S-ME at the agriculturally permitted concentration of 50 mL·hm^(-2) and the organosilicon additive cetylheptasiloxane at concentrations of 0.25%and 0.5%.The structure and diversity of the rhizosphere microbial community of red bean were analyzed using 16S rRNA sequencing technology to explore the effects of S-ME on the root microbial community in the presence of the additive.The results demonstrated that different application methods of S-ME and organosilicon additives significantly influenced the diversity of the rhizosphere microbial community in red bean.The sole application of S-ME led to a reduction in microbial diversity,particularly a significant decline in the abundance of beneficial microorganisms such as Acidobacteria(RB41).In contrast,the co-application with additives mitigated this inhibitory effect and promoted the growth of microorganisms such as Sphingomonas and Gaiella.Furthermore,the 0.5%organosilicon additive treatment significantly increased the abundance of Blastococcus and Stenotrophomonas,optimizing microbial community diversity.Co-occurrence network analysis also reflected the synergistic promotion effect of the additives.The study revealed that additives effectively alleviated the toxic effects of herbicides on rhizosphere microorganisms by regulating the rhizosphere microenvironment and promoting the growth of beneficial microorganisms.This research contributes to a deeper understanding of the regulatory mechanisms of additives on S-ME-induced changes in the rhizosphere microbial community of red bean,providing support for the rational application of herbicides and their additives in agricultural production.

关 键 词：精异丙甲草胺 有机硅助剂 红小豆 根际微生物 16S rRNA 

分 类 号：S482.4[农业科学—农药学] S154.3[农业科学—植物保护]