一株新型海洋噬藻体QB2的分离、基因组学分析及生物地理分布  

ISOLATION,WHOLE-GENOME ANALYSIS,AND BIOGEOGRAPHIC DISTRIBUTION OF A NOVEL MARINE CYANOPHAGE QB2

作　　者：苏畅 崔丹华 周洪艳 王天聪 王褚晓 陈鑫 邵红兵[1,2,3] 郭萃 汪岷[1,2,3,4] SU Chang;CUI Dan-Hua;ZHOU Hong-Yan;WANG Tian-Cong;WANG Chu-Xiao;CHEN Xin;SHAO Hong-Bing;GUO Cui;WANG Min(College of Marine Life Sciences,Key Laboratory of Evolution&Marine Biodiversity(Ministry of Education),Ocean University of China,Qingdao 266003,China;Institute of Evolution and Marine Biodiversity,Ocean University of China,Qingdao 266003,China;Frontiers Science Center for Deep Ocean Multispheres and Earth System,Ocean University of China,Qingdao 266003,China;Haide College,Ocean University of China,Qingdao 266003,China;Qingdao Eighth People’s Hospital,Qingdao 266121,China)

机构地区：[1]中国海洋大学海洋生命学院,海洋生物多样性与进化教育部重点实验室,山东青岛266003 [2]中国海洋大学海洋生物多样性与进化研究所,山东青岛266003 [3]中国海洋大学深海圈层与地球系统前沿科学中心,山东青岛266003 [4]中国海洋大学海德学院,山东青岛266003 [5]青岛市第八人民医院,山东青岛266121

出　　处：《海洋与湖沼》2025年第2期380-392,共13页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金项目,42176149号,41906126号,41976117号,42120104006号。

摘　　要：在南海海盆区分离得到一株侵染海洋聚球藻的噬藻体QB2,分析表明其基因组全长为171898bp,GC含量为41.49%。在噬藻体QB2基因组中检测到212个开放阅读框(ORFs)和7个tRNA基因。所预测到的ORFs根据功能可分为病毒粒子结构模块、DNA复制与调控模块、包装模块和辅助代谢功能模块。其携带有与光合作用、碳代谢、磷代谢、能量获取和应激调节等相关的20个辅助代谢基因,可能在调控噬藻体侵染过程中宿主的能量代谢方面发挥关键作用。系统发育分析表明噬藻体QB2与Kyanoviridae科Tefnutvirus属成员亲缘关系密切,且与该属成员S-IOM18的核苷酸同一性为80.3%,表明噬藻体QB2可被归类为Tefnutvirus属的新物种。噬藻体QB2的同源基因的生物地理分布分析表明,其主要分布在温热带海洋浅层区、温热带海洋中层区以及北极海区。本研究丰富了蓝藻噬藻体的基因组数据库,为进一步研究噬藻体的系统发育及其与宿主的相互作用提供了基础数据。A cyanophage QB2 that infects marine Synechococcus was isolated from an oligotrophic waters of the South China Sea basin.The complete genome of the QB2 was 171898 bp long,with a G+C content of 41.49%,from which 212 ORFs and 7 tRNAs were identified.The predicted ORFs were categorized into functional modules,including virus particle structural module,DNA and regulatory module,packaging module,and auxiliary metabolic genes.The QB2 carried 20 auxiliary metabolic genes that related to photosynthesis,carbon metabolism,phosphorus metabolism,energy acquisition,and stress regulation,and may play a key role in modulating the energy metabolism of the host during infection.Phylogenetic analysis shows that the QB2 was closely related to the members of the genus Tefnutvirus within the family Kyanoviridae,and had a nucleotide identity of 80.3%with Synechococcus phage S-IOM18,indicating that the QB2 qualified as a novel species in the genus Tefnutvirus.The biogeographic analysis of homologous genes of the cyanophage QB2 shows that it distributes in mainly the epipelagic and mesopelagic zones of temperate and tropical oceans,as well as the Arctic waters.This study enriched the gene database of cyanophages and provided basic data for further research into the phylogeny of cyanophages and their interactions with the hosts.

关 键 词：噬藻体 聚球藻 基因组分析 辅助代谢基因 生物地理分布 

分 类 号：Q939.48[生物学—微生物学]