The non-canonical proteome:a novel contributor to cancer proliferation  

作　　者：Jorge Ruiz-Orera Norbert Hübner 

机构地区：[1]Cardiovascular and Metabolic Sciences,Max Delbrück Center for Molecular Medicine in the Helmholtz Association(MDC),Berlin,Germany [2]DZHK(German Center for Cardiovascular Research),Partner Site Berlin,Berlin,Germany [3]Charité-Universitätsmedizin,Berlin,Germany [4]Helmholtz Institute for Translational AngioCardioScience(HI-TAC)of the Max Delbrück Center for Molecular Medicine in the Helmholtz Association(MDC)at Heidelberg University,Heidelberg,Germany.✉email:[e-mail]

出　　处：《Cell Research》2025年第3期155-156,共2页细胞研究(英文版)

摘　　要：The non-canonical proteome,comprising hundreds of unannotated peptides encoded by small open reading frames,significantly expands the functional landscape of our genome beyond conventional protein-coding genes.Recent work by Shi et al.published in Cell Research combines several detection techniques and functional validations to explore this uncharted territory,and shows that more than a thousand of these small peptides play significant roles in tumor cell growth,offering new insights into cancer biology.Advanced technologies such as ribosome profiling(Ribo-seq)1 and mass spectrometry have revealed that many non-coding RNAs harbor small open reading frames(sORFs)encoding microproteins and peptides that are typically shorter than 100 amino acids.2–4 Ribo-seq identifies actively translated sORFs by capturing and sequencing fragments of messenger RNA protected by ribosomes,providing a detailed view of nucleotide translation with high resolution.Ribo-seq does not directly detect proteins but provides evidence of ongoing translation.By contrast,mass spectrometry fragments proteins to measure their masses,making it possible to discover novel peptides encoded by sORFs.2,4 Unlike conventional“canonical”proteins annotated in databases,these small peptides have largely been overlooked and remain absent from major reference databases.

关 键 词：small peptides detection techniques cancer biologyadvan cell research mass spectrometry non canonical proteome ribo seq small open reading framessignificantly 

分 类 号：R730.2[医药卫生—肿瘤]