PBX3和C1QBP在喉癌组织中的表达及与预后的关系  

The expression of PBX3 and C1QBP in laryngeal cancer tissue and their relationship with prognosis

作　　者：刘彬 胡智慧 孔娜 李炎[3] LIU Bin;HU Zhihui;KONG Na;LI Yan(Department of Otolaryngology,Head and Neck Surgery,Shijiazhuang People's Hospital,Shijiazhuang 050000,China;Department of Oncology,Shijiazhuang People's Hospital,Shijiazhuang 050000,China;Department of Orthopaedics,The Third Hospital of Shijiazhuang,Shijiazhuang 050011,China)

机构地区：[1]石家庄市人民医院耳鼻咽喉头颈外科,河北石家庄050000 [2]石家庄市人民医院肿瘤一科,河北石家庄050000 [3]石家庄市第三医院骨科,河北石家庄050011

出　　处：《西部医学》2025年第4期532-537,共6页Medical Journal of West China

基　　金：河北省卫生健康委员会医学科学研究课题计划项目(20221638)。

摘　　要：目的研究喉癌组织中前B细胞白血病同源盒基因3(PBX3)和补体成分1q亚单位结合蛋白(C1QBP)的表达,探讨两者与临床预后的关系。方法选取自2018年1月—2020年1月石家庄市人民医院诊治的132例喉癌患者。采用实时荧光定量聚合酶链反应检测癌组织和癌旁组织PBX3、C1QBP mRNA表达。采用免疫组织化学法检测组织中PBX3、C1QBP蛋白表达。Pearson相关性分析癌组织中PBX3与C1QBP mRNA表达的相关性。比较不同临床病理特征喉癌患者癌组织PBX3、C1QBP表达。Kaplan-Meier曲线分析不同PBX3、C1QBP mRNA表达组喉癌患者3年生存率差异。COX回归分析影响喉癌患者预后的因素。结果癌组织中PBX3、C1QBP mRNA的相对表达量明显高于癌旁组织,差异具有统计学意义(t=46.692,55.185,均P<0.001)。CRC癌组织中PBX3、C1QBP阳性率高于癌旁组织,差异具有统计学意义(χ^(2)=96.458、77.633,均P<0.001)。癌组织PBX3与C1QBP mRNA表达呈明显正相关(r=0.712,P<0.001)。不同肿瘤TNM分期、肿瘤分化程度及淋巴结转移癌组织中PBX3、C1QBP mRNA表达比较,差异具有统计学意义(均P<0.001)。PBX3 mRNA高表达组和低表达组3年生存率为70.77%(46/65),92.65%(62/67),C1QBP mRNA高表达组和低表达组3年生存率为71.88%(46/64),91.18%(62/68)。PBX3、C1QBP mRNA高表达组3年累积生存率分别低于PBX3、C1QBP mRNA低表达组,差异具有统计学意义(Log-Rankχ^(2)=12.761、10.910,均P<0.001)。肿瘤TNM分期Ⅲ~Ⅳ期、淋巴结转移、PBX3 mRNA高表达、C1QBP mRNA高表达是影响喉癌患者不良预后的独立危险因素(均P<0.001)。结论喉癌中PBX3、C1QBP表达升高,两者与肿瘤TNM分期、肿瘤分化程度及淋巴结转移有关,是影响喉癌预后的独立危险因素。Objective To investigate the expression of pre-B-cell leukemia homeobox gene 3(PBX3)and complement C1q binding protein(C1QBP)in laryngeal cancer,and explore their relationship with clinical prognosis.Methods 132 patients with laryngeal cancer treated in our hospital from January 2018 to January 2020 were selected.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of PBX3 and C1QBP mRNA in cancer tissues and adjacent tissues.The expression of PBX3 and C1QBP protein in tissues was detected by immunohistochemistry.Pearson correlation analysis was used to analyze the correlation between PBX3 and C1QBP mRNA expression in cancer tissues.The expressions of PBX3 and C1QBP in laryngeal cancer tissues of patients with different clinicopathological characteristics were compared.Kaplan-Meier curve was used to analyze the differences in 3-year survival rate of patients with laryngeal cancer in different PBX3 and C1QBP mRNA expression groups.COX regression analysis was used to analyze the factors affecting the prognosis of patients with laryngeal cancer.Results The relative expression levels of PBX3 and C1QBP mRNA in cancer tissues were(3.72±0.58)and(3.11±0.43),which were significantly higher than(1.12±0.27)and(0.79±0.22)in adjacent tissues,and the difference was statistically significant(t=46.692,55.185,all P<0.001).The positive rates of PBX3 and C1QBP in CRC cancer tissues were 96.77%(60/62)and 91.94%(57/62),which were higher than 9.68%(6/62)and 12.90%(8/62)in adjacent tissues,and the differences were statistically significant(χ^(2)=96.458,77.633,all P<0.001).PBX3 mRNA expression was positively correlated with C1QBP mRNA expression(r=0.712,P<0.001).There were significant differences in the expression of PBX3 and C1QBP mRNA in tumor tissues of different TNM stages,tumor differentiation and lymph node metastasis(all P<0.001).The 3-year survival rates of PBX3 mRNA high expression group and low expression group were 70.77%(46/65)and 92.65%(62/67),and the 3-year survival rates of

关 键 词：喉癌 前B细胞白血病同源盒基因3 补体成分1q亚单位结合蛋白 预后 

分 类 号：R739.65[医药卫生—肿瘤]