Processing-induced reduction in dianthrones content and toxicity of Polygonum multiflorum:Insights from ultra-high performance liquid chromatography triple quadrupole mass spectrometry analysis and toxicological assessment  

作　　者：Wan-Fang Li Ying Wang Cai-Xia Qiu Jie Li Jie Bao Jian-Bo Yang Hong-Tao Jin 

机构地区：[1]New Drug Safety Evaluation Center,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing,China [2]Beijing Union-Genius Pharmaceutical Technology Development Co.,Ltd.,Beijing,China [3]NMPA Key Laboratory for Safety Research and Evaluation of Innovative Drug,Beijing,China [4]National Institutes for Food and Drug Control,Beijing,China

出　　处：《Animal Models and Experimental Medicine》2025年第4期685-695,共11页动物模型与实验医学(英文)

基　　金：National Natural Science Foundation of China,Grant/Award Number:81973476;Chinese Society of Toxicology,Grant/Award Number:CST2021CT101。

摘　　要：Background:Polygonum multiflorum-induced liver injury(PM-DILI)has significantly hindered its clinical application and development.Methods:This study investigates the variation in content and toxicity of dian-thrones,the toxic components of P.multiflorum,during different processing cycles.We employed the ultra-high-performance liquid chromatography triple quadrupole mass spectrometry method to quantify six dianthrones in raw P.multiflorum and formulations processed with a method called nine cycles of steaming and sunning.Additionally,toxicity assessments were conducted using human normal liver cell line L02 and zebrafish embryos.Results:Results indicate a gradual reduction in dianthrones content with increasing processing cycles.Processed formulations exhibited significantly reduced cytotoxic-ity in L02 cells and hepatotoxicity in zebrafish embryos.Conclusions:Our findings elucidate the relationship between processing cycles and P.multiflorum toxicity,providing theoretical support for its safe use.

关 键 词：dianthrones HEPATOTOXICITY Polygonum multiflorum Thunb. ultra-high performance liquid chromatography triple quadrupole mass spectrometry(UHPLC-QQQ-MS/MS) zebrafish embryos 

分 类 号：R284.1[医药卫生—中药学]