结直肠癌中TAK1表达与微卫星不稳定的相关性及生存率的关系  

The Correlation Between TAK1 Expression and Survival Rate in Colorectal Cancer

作　　者：朱丽娟[1] 李鹏 张欣 唐海明 ZHU Lijuan;LI Peng;ZHANG Xin;TANG Haiming(Department of Anorectal,Shanghai Hospital of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]上海市中医医院肛肠科,上海200071

出　　处：《标记免疫分析与临床》2025年第3期496-501,531,共7页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金青年项目(编号:82305230)。

摘　　要：目的探讨结直肠癌中TAK1表达与微卫星不稳定的相关性及生存率的关系。方法回顾性选取2017年5月至2019年6月就诊于本院并行结直肠癌根治术患者179例,收集患者的结直肠癌组织和癌旁组织,免疫组化SP法检测TAK1及MSI相关蛋白的表达,Spearman相关分析TAK1阳性表达与MSI的相关性,采用Kaplan-Meier生存曲线绘制TAK1表达结直肠癌患者的生存曲线,Cox回归分析结直肠癌患者预后影响因素。结果癌组织中TAK1阳性表达为65.36%,高于癌旁组织中TAK1阳性表达38.55%(P<0.001);癌组织中TAK1阳性表达与淋巴结转移、肿瘤分化、脉管侵袭、浸润深度相关(P<0.05);对微卫星不稳定结直肠癌(MSI)的相关蛋白MLH1、MSH2、MSH6和PMS2进行检测,判定为MSS组153例,发生率为85.47%,MSI组26例,发生率为14.53%;MSI共计26例。经Spearman相关分析结果显示,TAK1阳性表达与MSI呈负相关性(r=-0.403,P<0.001);在5年生存率中:患者TAK1阳性表达占45.30%(53/117),TAK1阴性表达占66.13%(41/62),TAK1阳性表达低于TAK1阴性表达(Log-rankχ2=6.966 P=0.008)。结论结直肠癌中TAK1阳性表达明显升高,与淋巴结转移、肿瘤分化、脉管侵袭、浸润深度相关,且与MSI呈负相关,可对预后的良好指标进行预测。Objective To investigate the correlation between TAK1 expression with survival rate in colorectal cancer.Methods A retrospective study was conducted among 179 patients who underwent concurrent radical resection for colorectal cancer at our hospital from May,2017 to June,2019.Cancer and adjacent tissues were collected from study subjects.The expressions of TAK1 and MSI were determined by SP method.Spearman correlation analysis was performed for associations of TAK1 positive and MSI expression.Kaplan Meier survival analysis was applied to plot the survival curve of TAK1 expression in colorectal cancer,and Cox regression analysis was used to cancer patient prognosis factors.Results The positive TAK1 in tissue was 65.36%,significantly higher than the negative expression of TAK1 in colorectal cancer tissue with 38.55%(P<0.001);The positive expression of TAK1 in tumor was significantly associated with differentiation degree,invasion depth,and vascular invasion(P<0.05);MLH1,MSH2,MSH6,and PMS2 related proteins of microsatellite unstable colorectal cancer(MSI)were detected,and 153 cases were identified as MSS group,with an incidence rate of 85.47%,and 26 cases as MSI group,with an incidence rate of 14.53%;There were a total of 26 cases of MSI,including 9 cases of TAK1 positive and 17 cases of negative;There were a total of 153 cases of cancer(MSS),among which 108 cases were positive for TAK1 and 45 cases were negative.Spearman analysis showed a negative correlation between TAK1 positive and MSI(r=-0.403,P<0.001).The 5-year survival rate of TAK1 positive expression patients was 45.30%(53/117),while the 5-year survival rate of TAK1 negative expression patients was 66.13%(41/62).The 5-year survival TAK1 positive was significantly lower than TAK1 negative patients(log rank chi square=6.966,P=0.008).Conclusion The positive TAK1 is significantly increased,and positively associated with the depth of invasion,lymph node metastasis,while negatively correlated with MSI.It can be used as a novel indicator for predicting prognosis.

关 键 词：结直肠癌 TAK1 微卫星不稳定 生存率 预后 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]