葡萄绿枝嫁接愈合过程中内源激素含量变化及转录组解析  

Changes of endogenous hormone concentrations and transcriptome analysis during the healing process of greenwood grafting in grape

作　　者：邓嘉漪 魏嘉彦 陈爱赛 何非[1] 于可济[1] 王军[1] DENG Jiayi;WEI Jiayan;CHEN Aisai;HE Fei;YU Keji;WANG Jun(Center for Viticulture and Enology,College of Food Science and Nutritional Engineering,China Agricultural University/Key Laboratory of Viticulture and Enology,Ministry of Agriculture and Rural Affairs,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院葡萄与葡萄酒研究中心·农业农村部葡萄酒加工重点实验室,北京100083

出　　处：《果树学报》2025年第4期732-751,共20页Journal of Fruit Science

基　　金：现代农业产业技术体系专项资金(CARS-29);宁夏回族自治区重点研发计划重大项目(2024BBF01002-03)。

摘　　要：【目的】研究葡萄绿枝嫁接愈合过程中嫁接接口(接穗和砧木结合部分)激素含量变化,结合转录组学数据分析,明确葡萄绿枝嫁接愈合过程中起关键作用的激素,同时为揭示葡萄嫁接愈合的分子机制提供参考。【方法】以天工丽人/贝达、天工墨玉/贝达和阳光玫瑰/贝达砧穗组合的嫁接接口为材料,分别在嫁接后1、4、7、10和13 d采集样品,采用高效液相色谱-三重四级杆串联质谱仪测定嫁接接口生长素(auxin,IAA)、异戊烯基腺嘌呤(isopentenyladenine,IP)、脱落酸(abscisic acid,ABA)和茉莉酸类物质(jasmonates,JAs)的含量。同时对天工丽人/贝达的嫁接接口进行转录组测序。【结果】葡萄绿枝嫁接愈合过程中IAA含量呈上升趋势,而IP、ABA、JAs含量总体呈下降趋势。转录组分析显示27个差异表达基因参与植物激素合成和代谢,49个差异表达基因参与植物激素信号转导。其中GH3(Vitvi03g00586)、LOX(Vitvi06g00158)、AOS(Vitvi18g00886)、OPCL1(Vitvi18g00124)、ABF(Vitvi12g01667)、TCH4(Vitvi11g01682)的表达量与JA、IP含量呈显著正相关;AHA(Vitvi14g01888)、TIR1(Vitvi07g00248)的表达量与ABA、IP含量呈显著负相关,说明以上基因可能是愈合过程中与内源激素相关的关键基因。【结论】葡萄绿枝嫁接愈合过程中,植物激素的含量变化具有阶段特异性,IAA在嫁接愈合过程中起关键作用,IP、ABA、JAs通过与IAA信号转导相互作用,共同调控绿枝嫁接愈合。【Objective】To study the changes of hormone concentrations in the graft union during the healing process of greenwood grafting,the experiment was carried out,combined with transcriptomic data analysis,so as to identify the key hormone changes in the healing process of greenwood grafting in grape,and provide references for revealing the molecular mechanism of the healing process.【Methods】The stion combinations of Tiangong Liren/Beta,Tiangong Moyu/Beta and Shine Muscat/Beta were used as materials and were collected at 1,4,7,10 and 13 days after grafting(DAG),respectively.The concentrations of auxin(IAA),isopentenyladenine(IP),abscisic acid(ABA)and jasmonates(JAs)in the graft union were determined by high performance liquid chromatograph-triple quadrupole tandem mass spectrometry(HPLC-QqQ-MS/MS).Simultaniously,transcriptome sequencing was performed on the graft union of Tiangong Liren/Beta.【Results】From the perspective of morphology,Tiangong Moyu/Beta buds burst first,and the bud swelling was observed at 4 DAG.Tiangong Liren/Beta and Shine Muscat/Beta were observed to have swollen buds at 7 DAG.Different stion combinations com pleted the healing process within 13 DAG.From the analysis of plant hormone levels,the concentration of IAA increased,while the concentrations of IP,ABA and JAs decreased during healing.From transcriptome level analysis,the transcriptome data of Tiangong Liren/Beta at different stages were pairwise compared,and 6319 differentially expressed genes were screened.1 DAG vs 13 DAG had the largest number of differentially expressed genes,with 3846,the second was 1 DAG vs 10 DAG,with 3301 and the third was 1 DAG vs 7 DAG,with 2968.The results showed that the gene transcriptional regulation of 7 DAG,10 DAG and 13 DAG were significantly changed compared with 1 DAG.The 10 DAG vs 13 DAG comparison combination had the smallest number of differentially expressed genes,indicating that there was little difference in the gene expression pattern between 10 DAG and 13 DAG.6319 differentially expresse

关 键 词：葡萄 绿枝嫁接 内源激素 转录组 差异表达基因 愈合过程 

分 类 号：S663.1[农业科学—果树学]