手性纳米金改性TiO_(2)对番茄灰霉菌的光催化抑菌效果  

Photocatalytic bactericidal effect of chiral gold-modified TiO_(2)nanoparticles on Botrytis cinerea in tomatoes

作　　者：邹洁[1,2] 宋锦柱 郭星磊[3] 李昕炫 倪永标 严文静 ZOU Jie;SONG Jinzhu;GUO Xinglei;LI Xinxuan;NI Yongbiao;YAN Wenjing(Jiangsu Product Quality Supervision and Inspection Research Institute,Nanjing,Jiangsu 210007,China;Key Laboratory of the State Administration of Market Supervision and Administration(Food Contact Material Safety),Nanjing,Jiangsu 210007,China;College of Food Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China)

机构地区：[1]江苏省产品质量监督检验研究院,江苏南京210007 [2]国家市场监督管理总局重点实验室(食品接触材料安全),江苏南京210007 [3]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品与机械》2025年第3期26-32,共7页Food and Machinery

基　　金：江苏省市场监督管理局科技计划项目(编号:KJ2024007)。

摘　　要：[目的]开发适用于番茄中灰霉菌的抑菌方法。[方法]以番茄灰霉菌为试验对象,通过菌落直径、抑菌率考察手性纳米金改性TiO_(2)(DAT)复合材料中纳米材料添加量、光照时间对灰霉菌的抑制效果;通过分析菌丝生物量、核酸蛋白质泄漏量及菌体结构探究DAT对灰霉菌的作用机理。[结果]当手性纳米金添加量为80μL、DAT质量浓度为5 mg/mL、自然光照时间为24 h时,DAT对灰霉菌的抑菌率最高为53.64%;DAT纳米材料在光照中产生的自由基通过破坏细胞膜,导致菌体核酸和蛋白质大量泄露,从而抑制灰霉菌菌丝的生长;DTA处理后的灰霉菌菌丝表面粗糙,出现皱缩、破损等情况。[结论]DTA可以有效破坏番茄灰霉菌细胞膜及菌丝结构,抑制灰霉菌的生长繁殖。[Objective]To develop an effective method for inhibiting Botrytis cinerea in tomatoes.[Methods]The effect of the addition amount of nanomaterial and light exposure time in chiral gold-modified TiO_(2)(DAT)composite materials on the inhibition of B.cinerea in tomatoes was studied through colony diameter and antibacterial rate.The mechanism of action of DAT on B.cinerea was explored by analyzing mycelial biomass,nucleic acid and protein leakage,and fungal cell structure.[Results]When the chiral nano-gold addition was 80μL,the DAT concentration was 5 mg/mL,and the natural light exposure time was 24 hours,the highest inhibition rate of DAT against B.cinerea was 53.64%.DAT nanomaterials generated free radicals under light exposure,disrupting the cell membrane,causing extensive leakage of fungal nucleic acids and proteins,and inhibiting the growth of B.cinerea mycelia.The surfaces of B.cinerea mycelia treated with DAT were rough,wrinkled,and damaged,effectively destroying the mycelial structure of B.cinerea.[Conclusion]DAT can effectively destroy the cell membrane and mycelial structure of B.cinerea in tomatoes,inhibiting the growth and reproduction of B.cinerea.

关 键 词：手性 金纳米 二氧化钛 灰霉菌 抑菌 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]