miR-183对前列腺癌细胞增殖的影响  

Effect of MiR-183 on Proliferation of LNCaP Cells in Prostate Cancer

作　　者：戴研平 杨建辉[2] 杨喆 DAI Yan-ping;YANG Jian-Hui;YANG Zhe(Yueyang Vocational Technical College Medical School,Yueyang,Hunan 414000,China;Qiannan Medical College for Nationalities,Duyun,Guizhou 558013,China;Basic Medical College,Guizhou Medical University,Guiyang,Guizhou 550000,China)

机构地区：[1]岳阳职业技术学院医学院,湖南岳阳414000 [2]黔南民族医学高等专科学校,贵州都匀558013 [3]贵州医科大学基础医学院,贵州贵阳550000

出　　处：《黔南民族医专学报》2025年第1期1-4,共4页Journal of Qiannan Medical College for Nationalities

摘　　要：目的:探讨miR-183对人前列腺癌细胞增殖能力的影响。方法:采用实时荧光定量聚合酶链式反应(RT-qPCR)检测分别用miR-183-mimic和miR-183-inhibitor过表达和抑制miR-183后LNCaP细胞株和雄激素非依赖性前列腺癌PC3细胞株中miR-183表达;采用CCK-8法检测前列腺癌细胞的增殖能力;Western blot实验检测PCa人前列腺癌雄激素依赖性LNCaP中TMP1蛋白表达水平的变化。结果:RT-qPCR结果表明过表达前列腺癌miR-183后细胞中miR-183表达显著升高,反之miR-183-inhibitor处理后细胞中miR-183表达下降,说明外源转录的miR-183-mimic和miR-183-inhibitor能够增加或抑制miR-183的表达水平;CCK-8实验结果显示,与NC组相比,miR-183-mimic组细胞增殖显著升高,miR-183-inhibitor组细胞增殖显著降低(P<0.01)。Western blot实验结果显示过表达miR-183后,TMP1的蛋白表达水平显著降低,抑制miR-183后,TMP1的蛋白表达水平显著升高(P<0.01)。结论:过表达miR-183可促进PCa细胞株LNCaP的增殖,敲低miR-183可抑制PCa细胞株LNCaP的增殖。Objective:To investigate the effect of miR-183 on the proliferation ability of human prostate cancer cells.Methods:Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of miR-183 in LNCaP cell line and androgen-independent prostate cancer PC3 cell line after overexpression and inhibition of miR-183 with miR-183-mimic and miR-183-inhibitor,respectively;The proliferation ability of prostate cancer cells was detected by CCK-8 assay;Western blot was used to detect the changes in the expression level of TMP1 protein in androgen-dependent LNCaP cells of human prostate cancer.Results:The results of RT-qPCR showed that the expression of miR-183 in cells overexpressing prostate cancer miR-183 significantly increased,while the expression of miR-183 in cells treated with miR-183-inhibitor decreased,indicating that exogenous transcription of miR-183-mimic and miR-183-inhibitor can increase or inhibit the expression level of miR-183;The CCK-8 assay results showed that the cell proliferation in the miR-183-mimic group was significantly higher than that in the NC group,while the cell proliferation in the miR-183-inhibitor group was significantly lower than that in the NC group(P<0.01).Western blot analysis showed that the protein expression level of TMP1 significantly decreased after overexpression of miR-183,and significantly increased after inhibition of miR-183(P<0.01).Conclusion:Overexpression of miR-183 can promote the proliferation of PCa cell line LNCaP,while knockdown of miR-183 can inhibit the proliferation of PCa cell line LNCaP.

关 键 词：前列腺癌 miR-183 增殖 TMP1 

分 类 号：R737.25[医药卫生—肿瘤]