LncRNA TUG1调控Wnt/β-catenin通路对人牙髓干细胞成牙本质分化的影响  

Effects of lncRNA TUG1 regulating Wnt/β-catenin pathway on the dentin differentiation of human dental pulp stem cells

作　　者：刘铮 刘沛 刘雅贞 LIU Zheng;LIU Pei;LIU Yazhen(Department of Dental Pulp,Xining Stomatological Hospital,Xining Qinghai 810000;Department of Stomatology,The Third People′s Hospital of Henan,Zhengzhou Henan 450018,China)

机构地区：[1]青海省西宁市口腔医院牙体牙髓科,810000 [2]河南省直第三人民医院口腔科,河南郑州450018

出　　处：《蚌埠医科大学学报》2025年第3期293-297,共5页Journal of Bengbu Medical University

摘　　要：目的:研究长链非编码核糖核酸牛磺酸上调基因1(lncRNA TUG1)通过调控无翅型MMTV整合位点家族成员蛋白/β-连环素(Wnt/β-catenin)通路对人牙髓干细胞(hDPSCs)成牙本质分化的影响。方法:体外分离培养hDPSCs,分为空白对照组、阴性对照组(siRNA-NC组)、沉默TUG1表达组(TUG1-siRNA组)、联合组(TUG1-siRNA+Wnt/β-catenin通路激活剂氯化锂)。采用实时荧光定量PCR法检测TUG1水平表达情况;CCK-8法检测hDPSCs增殖能力;茜素红染色法检测成牙本质诱导分化效果;蛋白免疫印迹法检测成牙本质分化相关蛋白[人神经菌毛素1(NRP1)、牙本质涎蛋白(DSPP)、牙本质基质蛋白1(DMP1)]及Wnt、β-catenin表达水平。结果:与空白对照组、siRNA-NC组比较,TUG1-siRNA组hDPSCs增殖抑制率升高,hDPSCs深红色钙化结节形成能力、NRP1、DSPP、DMP1、Wnt、β-catenin蛋白表达均降低(P<0.05)。与TUG1-siRNA组比较,联合组hDPSCs增殖抑制率降低,hDPSCs深红色钙化结节形成能力、NRP1、DSPP、DMP1、Wnt、β-catenin蛋白表达均升高(P<0.05)。结论:沉默lncRNA TUG1可能通过抑制Wnt/β-catenin信号通路活化抑制hDPSCs增殖及成牙本质分化。Objective:To study the effect of long non-coding ribonucleic acid taurine up-regulated gene 1(lncRNA TUG1)on dentin differentiation of human dental pulp stem cells(hDPSCs)by regulating the wingless-type MMTV integration site family/β-catenin(Wnt/β-catenin)pathway.Methods:HDPSCs were isolated and cultured in vitro,and divided into the blank control group,negative control group(siRNA-NC group),silent TUG1 expression group(TUG1-siRNA group)and combined group(TUG1-siRNA+activation agent of Wnt/β-catenin pathway lithium chloride).The real-time fluorescence quantitative PCR was used to detect the expression of TUG1;the proliferation ability of hDPSCs was detected by CCK-8;alizarin red staining method was used to to detect the differentiation effect of dentin,and the expression levels of dentin differentiation-related proteins[human neuropilin 1(NRP1),dentin matrix protein(DMP1),dentin sialoprotei(DSPP)],Wnt andβ-catenin proteins were detected by Western blotting.Results:Compared with the blank control group and siRNA-NC group,the proliferation inhibition rate of hDPSCs significantly increased,and the dark red calcified nodules forming ability and expression levels of NRP1,DSPP,DMP1,Wnt,andβ-catenin protein significantly reduced in the TUG1-siRNA group(P<0.05).Compared with the TUG1-siRNA group,the proliferation inhibition rate of hDPSCs significantly reduced,and the dark red calcified nodules forming ability and expression levels of NRP1,DSPP,DMP1,Wnt andβ-catenin proteins significantly increased in the combined group(P<0.05).Conclusions:Silencing lncRNA TUG1 may inhibit the proliferation of hDPSCs and dentin differentiation by inhibiting the activation of Wnt/β-catenin signaling pathway.

关 键 词：人牙髓干细胞 长链非编码核糖核酸牛磺酸上调基因1 成牙本质分化 

分 类 号：R781[医药卫生—口腔医学]