circ_0061140靶向miR-548b-3p调控胃癌细胞增殖、周期分布和凋亡的机制研究  

Study on the the mechanism of circ_0061140 regulating the proliferation,cycle distribution and apoptosis of gastric cancer cells by targeting miR-548b-3p

作　　者：牛涛 庄唯 伍苏娟 NIU Tao;ZHUANG Wei;WU Sujuan(Department of Gastroenterology,Wuhan Dongxihu District People′s Hospital,Wuhan Hubei 430040,China)

机构地区：[1]湖北省武汉市东西湖区人民医院消化内科,430040

出　　处：《蚌埠医科大学学报》2025年第3期298-303,308,共7页Journal of Bengbu Medical University

摘　　要：目的:研究胃癌细胞中circ_0061140和miR-548b-3p表达水平,探讨circ_0061140靶向miR-548b-3p对胃癌细胞增殖、周期分布和凋亡的影响。方法:RT-qPCR检测人胃黏膜细胞GES1和胃癌细胞系(SNU-1、AGS、HS-746T)中circ_0061140和miR-548b-3p相对水平。将circ_0061140的小干扰RNA、miR-548b-3p模拟物、circ_0061140的小干扰RNA+miR-548b-3p抑制物分别转染到SNU-1细胞,通过CCK-8实验检测SNU-1细胞活力,通过流式细胞仪检测细胞凋亡和周期分布。采用双荧光素酶报告实验、RIP实验和RT-qPCR分析circ_0061140和miR-548b-3p靶向调控关系。结果:与GES1细胞比较,SNU-1、AGS、HS-746T细胞中circ_0061140相对水平升高(P<0.05~P<0.01),miR-548b-3p相对水平降低(P<0.05~P<0.01)。干扰circ_0061140表达后SNU-1细胞活力、S期比例降低(P<0.05),凋亡率、G0/G1期比例、miR-548b-3p相对水平升高(P<0.05)。过表达miR-548b-3p后SNU-1细胞活力、S期比例降低(P<0.01),凋亡率、G0/G1期比例升高(P<0.01)。过表达circ_0061140后SNU-1细胞miR-548b-3p相对水平降低(P<0.05)。circ_0061140与miR-548b-3p直接特异性结合。抑制miR-548b-3p表达明显减弱干扰circ_0061140对SNU-1细胞活力、周期分布以及凋亡的影响(P<0.01)。结论:胃癌细胞中circ_0061140表达上调,miR-548b-3p表达下调。干扰circ_0061140通过上调miR-548b-3p能够抑制胃癌细胞增殖,使细胞阻滞在G0/G1期,并诱导细胞凋亡。Objective:To investigate the expression levels of circ_0061140 and miR-548b-3p in gastric cancer cells,and explore the effects of circ_0061140 on the proliferation,cycle distribution and apoptosis of gastric cancer cells by targeting miR-548b-3p.Methods:The relative levels of circ_0061140 and miR-548b-3p in human gastric mucosal cells GES1 and gastric cancer cell lines(SNU-1,AGS,HS-746T)were detected using the RT-qPCR.The small interfering RNA of circ_0061140,miR-548b-3p mimic,and small interfering RNA of circ_0061140+miR-548b-3p inhibitor were transfected into SNU-1 cells,respectively.The viability of SNU-1 cells was detected by CCK-8 assay,the apoptosis and cycle distribution were detected by flow cytometry.The dual luciferase reporter experiment,RIP assay and RT-qPCR were used to analyze the target regulation relationship between circ_0061140 and miR-548b-3p.Results:Compared with GES1 cells,the relative level of circ_0061140 in SNU-1,AGS and HS-746T cells significantly increased(P<0.05),and the relative level of miR-548b-3p significantly decreased(P<0.05 to P<0.01).After interfering the expression of circ_0061140,the cell viability and S phase ratio of SNU-1 cells were significantly reduced(P<0.05 to P<0.01),and the apoptosis rate,G0/G1 phase ratio,and relative levels of miR-548b-3p significantly increased(P<0.05 to P<0.01).After overexpression of miR-548b-3p,the cell viability and S phase ratio of SNU-1 cells were significantly reduced(P<0.01),and the apoptosis rate and G0/G1 phase ratio significantly increased(P<0.01).The relative level of miR-548b-3p in SNU-1 cells was significantly reduced after overexpression of circ_0061140(P<0.05).The circ_0061140 was directly and specifically bound to miR-548b-3p.Inhibiting the miR-548b-3p expression could significantly reduce the effects of interfering circ_0061140 on the cell viability,cycle distribution and apoptosis of SNU-1 cells(P<0.01).Conclusions:The circ_0061140 expression is up-regulated,and the miR-548b-3p expression is down-regulated in gastric cancer cells

关 键 词：胃肿瘤 细胞增殖 细胞周期 凋亡 circ_0061140 miR-548b-3p 

分 类 号：R735.2[医药卫生—肿瘤]