LINC00339调控miR-214-3p对HPV16阳性宫颈癌细胞增殖、迁移及侵袭的影响  

Impacts of LINC00339 on the proliferation,migration,and invasion of HPV16 positive cervical cancer cells by modulating miR-214-3p

作　　者：曾彬彬 卢丹[2] 商潇予 朱佳宁 李佳璐 奚菊群 ZENG Binbin;LU Dan;SHANG Xiaoyu;ZHU Jianing;LI Jialu;XI Juqun(Institute of Translational Medicine,Yangzhou University School of Medicine,Yangzhou,Jiangsu 225001,China;Subei People's Hospital Affiliated toYangzhou University)

机构地区：[1]扬州大学医学院(转化医学研究院),江苏扬州225001 [2]扬州大学附属苏北人民医院

出　　处：《中国病原生物学杂志》2025年第5期557-562,共6页Journal of Pathogen Biology

基　　金：江苏省科技项目(No.BK20231329)。

摘　　要：目的探讨LINC00339调控miR-214-3p对HPV16阳性宫颈癌细胞增殖、迁移及侵袭的影响。方法qRT-PCR法检测HPV16阳性宫颈癌组织及细胞中LINC00339、miR-214-3p水平;将HPV16阳性宫颈癌细胞SiHa分别转染相应质粒后分为si-NC组、si-LINC00339组、miR-NC、miR-214-3p mimics、si-LINC00339+anti-NC组、si-LINC00339+anti-miR-214-3p组;qRT-PCR法检测各组SiHa细胞中LINC00339、miR-214-3p水平;Edu、划痕实验与Transwell实验分别检测细胞增殖、迁移、侵袭;Western blot检测增殖、迁移、侵袭相关蛋白表达;裸鼠移植瘤观察移植瘤生长情况;qRT-PCR法检测瘤组织LINC00339、miR-214-3p水平;免疫组化检测瘤组织增殖、迁移、侵袭相关蛋白表达。结果在宫颈癌组织及SiHa、CaSki细胞中LINC00339水平升高,miR-214-3p水平降低(P<0.05);沉默LINC00339可降低LINC00339水平及Edu阳性率、划痕愈合率、细胞侵袭数、Ki-67、PCNA、CD147、MMP-9表达,升高miR-214-3p水平(P<0.05);miR-214-3p过表达可降低Edu阳性率、划痕愈合率、细胞侵袭数、Ki-67、PCNA、CD147、MMP-9表达,升高miR-214-3p水平(P<0.05);沉默LINC00339后沉默miR-214-3p可升高Edu阳性率、划痕愈合率、细胞侵袭数、Ki-67、PCNA、CD147、MMP-9表达,降低miR-214-3p水平(P<0.05);Starbase网站预测及双荧光素酶实验报告显示LINC00339与miR-214-3p之间存在靶向关系;裸鼠移植瘤实验结果显示,沉默LINC00339可降低LINC00339水平,升高miR-214-3p水平,抑制瘤组织中Ki-67、PCNA、CD147、MMP-9表达,抑制裸鼠移植瘤生长(P<0.05)。结论LINC00339可通过靶向抑制miR-214-3p促进宫颈癌细胞增殖、迁移与侵袭。Objective To discuss the impacts of LINC00339 on proliferation,migration,and invasion of HPV16 positive cervical cancer cells by modulating miR-214-3p.Methods QRT-PCR was used to measure LINC00339 and miR-214-3p in HPV16 positive cervical cancer tissues and cells.HPV16 positive cervical cancer cells SiHa were transfected with corresponding plasmids and grouped into si-NC group,si-LINC00339 group,miR-NC group,miR-214-3p mimics group,si-LINC00339+anti-NC group,and si-LINC00339+anti-miR-214-3p group.QRT-PCR was used to test LINC00339 and miR-214-3p in SiHa cells.Edu,scratch assay and Transwell assay were applied to test cell proliferation,migration,and invasion,respectively.Western blot was applied to detect proliferation,migration,and invasion related proteins.The nude mouse transplant tumor was used to observe the growth of the transplant tumor.QRT-PCR was used to measure LINC00339 and miR-214-3p in tumor tissue.Immunohistochemistry was used to detect proteins related to tumor tissue proliferation,migration,and invasion.Results The LINC00339 increased and miR-214-3p decreased in cervical cancer tissues and SiHa,CaSki cells(P<0.05).Silencing LINC00339 could reduce LINC00339,Edu positivity rate,scratch healing rate,cell invasion number,Ki-67,PCNA,CD147,MMP-9,and raise miR-214-3p(P<0.05).Overexpression of miR-214-3p could decline Edu positivity rate,scratch healing rate,cell invasion number,Ki-67,PCNA,CD147,MMP-9,and raise miR-214-3p(P<0.05).Silencing miR-214-3p after silencing LINC00339 could increase Edu positivity rate,scratch healing rate,cell invasion number,Ki-67,PCNA,CD147,MMP-9,and reduce miR-214-3p(P<0.05).The Starbase website prediction and dual luciferase assay report indicated a targeted relationship between LINC00339 and miR-214-3p.Nude mouse transplantation experiments found that silencing LINC00339 could decline LINC00339,increase miR-214-3p,inhibit Ki-67,PCNA,CD147,MMP-9 in tumor tissue,and suppress the growth of nude mouse transplantation tumors(P<0.05).Conclusion LINC00339 can promote the proliferat

关 键 词：LINC00339 miR-214-3p HPV16阳性宫颈癌 增殖 迁移 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]