1株副猪嗜血杆菌分离鉴定及耐药性分析  

Isolation,identification and antimicrobial susceptibility of a strain of Haemophi-lus parasuis

作　　者：刘茜 王庚 林正丹 孙秀秀 金鑫鑫 李丽 杨俊杰 胡薛英[1] 谷长勤[1] 张万坡[1] 刘晓丽[1] 余腾 程国富[1,2] LIU Xi;WANG Geng;LIN Zhengdan;SUN Xiuxiu;JIN Xinxin;LI Li;YANG Junjie;HU Xue ying;GU Changqin;ZHANG Wanpo;LIU Xiaoli;YU Teng;CHENG Guofu(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;Guangri YangriangCo.,Ltd.,Guigang,Guangxi537100,China)

机构地区：[1]华中农业大学动物医学院,湖北武汉430070 [2]广西扬翔股份有限公司,广西贵港537100

出　　处：《中国兽医学报》2025年第2期219-226,共8页Chinese Journal of Veterinary Science

基　　金：中央高校基本科研业务费专项基金资助项目(140422008);扬翔股份有限公司重大创新变革资助项目(YXNM-R&D2022-02)。

摘　　要：猪关节炎作为规模化猪场常见的慢性疾病之一,会导致肉猪生产性能降低,对猪场的正常生产具有较大影响。本试验从1头跛行的猪关节液内分离纯化得到1株副猪嗜血杆菌(Haemophilus parasuis,HPS),对分离菌株进行血清型、毒力基因和耐药性等病原学方面的研究,结合敏感药物对临床关节炎肉猪展开治疗,为将来实际生产中肉猪关节病的综合防治提供理论依据。本试验从病猪关节液里分离得到1株14型强毒株HPS,HPS分离株对β-内酰胺类药物和四环素类药物敏感,氟苯尼考与多黏菌素在较低的药物浓度时也能完全抑制HPS分离株的生长,但细菌对新诺明和环丙沙星出现耐药性。使用盐酸多西环素注射液和恩诺沙星注射液对临床关节炎的病猪进行治疗,治疗效果良好。相较于感染组病猪腕关节、跗关节难以屈曲,明显跛行的状态,治疗组病猪明显好转,可正常行走,关节仅轻微肿胀,关节病临床症状得到缓解。Porcine arthritis,one of the common chronic diseases in large-scale pig farms,can signifi-cantly reduce the production performance of meat pigs.In this study,a strain of Haemophilus pa-rasuis(HPS)was isolated from the joint fluid of a lame pig.The HPS was analyzed in terms of se-rotypes,virulence genes,and resistance genes.Additionally,it was treated with sensitive antibiotics to provide a theoretical basis for the comprehensive prevention and treatment of arthritis in meat pigs in future production settings.A strain of HPS type 14 was isolated from the joint fluid of dis-eased pigs.The HPS isolate demonstrated sensitivity toβ-lactams and tetracyclines,while florfeni-col and polymyxin effectively inhibited its growth at low concentrations.However,the bacteria ex-hibited resistance to sulfonamides and ciprofloxacin.The treatment of affected pigs with clinical ar-thritis using doxycycline and enrofloxacin injections proved effective.Compared to the infected group,in which the sick pigs experienced difficulty flexing their carpal and tarsal joints and exhibi-ted significant lameness,the pigs in the treatment group showed marked improvement.Their joints were only slightly swollen,and the clinical symptoms of arthropathy were alleviated.

关 键 词：关节炎 HPS PCR鉴定 猪 

分 类 号：S858.28[农业科学—临床兽医学]