日粮中添加植物乳杆菌LP12通过调节肠道菌群预防肥胖  

Dietary supplementation of Lactiplantibacillus plantarum LP12 prevents obesity via regulating intestinal flora

作　　者：叶丹妮 邓玲聪 艾雪言 董雨 俞佳钰 郝嘉翼 李明玉 陈文聪 陈嘉豪 王子仪 白杰英 王茂鹏 YE Danni;DENG Lingcong;AI Xueyan;DONG Yu;YU Jiayu;HAO Jiayi;LI Mingyu;CHEN Wencong;CHEN Jiahao;WANG Ziyi;BAI Jieying;WANG Maopeng(Wenzhou Key Laboratory of Virology and Immunology,Institute of Virology,Wenzhou University,Wenzhou,Zhejiang 325005,China;College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210046,China;College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225000,China;College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanci 712000,China;Jilin Genet-Med Biotechnological Co.,Ltd.,Changchun 130122,China;Genet-Med(Dezhou)Biotechnological Co.,Ltd.,Dezhou,Shandong 253512,China;National Biomedical Imaging Center,Beijing University,Beijing 101408,China)

机构地区：[1]温州大学病毒学研究所温州市病毒学和免疫学重点实验室,浙江温州325005 [2]南京农业大学动物医学院,江苏南京210046 [3]扬州大学兽医学院,江苏扬州225000 [4]西北农林科技大学动物医学院,陕西杨凌712000 [5]吉林省金泰美迪生物技术有限公司,吉林长春130122 [6]金泰美迪(德州)生物技术有限公司,山东德州253512 [7]北京大学国家生物医学成像科学中心,北京101408

出　　处：《中国兽医学报》2025年第3期611-618,共8页Chinese Journal of Veterinary Science

基　　金：温州市科技特派员专项资助项目(X2023085);国家生猪技术创新中心先导科技资助项目(NTCIP-XD/B11);温州市农业丰收计划资助项目(FSJH2022003);国家自然科学基金青年基金资助项目(31802224)。

摘　　要：在日粮中添加植物乳杆菌LP12进行饲喂,观察并测定日本大耳兔的体质量增长和采食量情况,分析兔血液生化指标,以及血清和肝脏的抗氧化指标;通过16S测序分析菌群结构和丰度变化。结果显示,植物乳杆菌LP12能够显著抑制兔体质量增长,降低血清葡萄糖和谷丙转氨酶水平,增加肝脏的超氧化物歧化酶(superoxide dismutase,SOD)活力。16S菌群结构分析显示,在门水平,添加植物乳杆菌LP12能够增加拟杆菌门和脱硫杆菌门丰度;在属水平,添加植物乳杆菌LP12能够增加Muribaculaceae菌丰度。菌群功能预测分析发现,添加植物乳杆菌LP12对铁-硫簇和Zn依赖性蛋白酶具有正向调节作用。结果表明,添加植物乳杆菌能够抑制日本大耳兔体质量增加,增加肝脏抗氧化活性,且能够改变日本大耳兔的肠道菌群结构。This study aims to investigate the effect of Lactiplantibacillus plantarum LP12 on obesity prevention.In our study,Lac tip lantibacillus plantarum LP12 was added to the diet for feeding,and the blood biochemistry status of rabbit,as well as the antioxidant effect of serum and liver samples were analyzed by determining the body weight change and feed intake of Japanese White rabbits.The changes in colony structure and abundance were also analyzed by 16S rDNA sequencing.The results showed that supplementation of Lactiplantibacillus plantarum LP12 inhibits weight gain,decreases serum glucose and ALT levels,and increases SOD activity in the liver.16S RNA gene sequencing analysis showed that the addition of Lactiplantibacillus plantarum LP12increases the abundance of Bacteroidetes and Desulfovibrioides at the phylum level,and the supplementation of Lactiplantibacillus plantarum LP12 increases the abundance of Muribaculaceae at the genus level.Predictive analysis of microbiota function revealed that the supplementation of Lactiplantibacillus plantarum LP12 positively regulated iron-sulfur clusters and Zn-dependent proteases.In conclusion,the addition of Lactiplantibacillus plantarum effectively inhibits weight gain in Japanese White rabbits,enhances the antioxidative activity of the liver,and induces alterations in the gut microbiota composition of these rabbits.These findings lay an experimental foundation for further exploring the mechanisms by which Lactobacillus plantarum LP12 exerts its preventive effects against obesity and promotes metabolic health.

关 键 词：植物乳杆菌 肥胖 肠道菌群 血液生化 抗氧化指标 菌群功能 

分 类 号：S852.2[农业科学—基础兽医学] S852.61[农业科学—兽医学]