羊栖菜多糖抑制人肺癌细胞H1299血管生成拟态的作用  

The inhibitory effect of Sargassum fusiforme polysaccharides on vasculogenic mimicry in human lung cancer cell H1299

作　　者：陈慧玲[1] 龙香娥[1] 张玲[1] 万勇[1] 况炜[1] CHEN Huiling;LONG Xiange;ZHANG Ling;WAN Yong;KUANG Wei(Ningbo College of Health Scienees,Ningbo 315104,Zhejiang,China)

机构地区：[1]宁波卫生职业技术学院,宁波315104

出　　处：《现代实用医学》2025年第4期346-349,F0003,共5页Modern Practical Medicine

基　　金：宁波市科技计划项目(2021S147);浙江省教育厅一般科研项目(Y202250865)。

摘　　要：目的探讨羊栖菜多糖(SFPS)抑制人肺癌细胞H1299血管生成拟态(VM)的作用。方法采用CCK-8方法检测不同浓度SFPS(0、10、30、100、300及1000 mg/L)作用的H1299细胞活性,应用三维细胞培养技术分析不同浓度SFPS对H1299细胞VM的影响,并通过实时荧光定量PCR法测定H1299细胞中血管内皮生长因子-A(VEGF-A)及胞内磷脂酰肌醇激酶(PI3K)的mRNA表达水平。结果与对照组(SFPS浓度0 mg/L)相比,SFPS作用48 h后,H1299细胞存活率下降最为明显,30、100、300及1000 mg/L浓度组与对照组差异均有统计学意义(均<0.05)。随着SFPS处理浓度的增加,30、100及300mg/L浓度组VM管道结构数量较对照组均明显减少(均<0.05),并呈剂量依赖性。RT-PCR结果显示,不同浓度SFPS能够抑制H1299细胞中VEGF-A及PI3K的mRNA表达(均<0.05)。结论SFPS可降低人肺癌细胞H1299存活率,并抑制VM形成,这可能与下调VEGF-A与PI3K的表达有关。Objective To investigate the effect of Sargassum fusiforme polysaccharides(SFPS)on vasculogenic mi-micry(VM)in human lung cancer cell line H1299.Methods The cell line H1299 was treated with different concen-trations of SFPS(0,10,30,100,300,1000 mg/L),and the cell viability was detceted by cell count kit-8(CCK-8).The effect of different concentrations of SFPS on VM in H1299 cells was analyzed by three dimensional cell culture technology.The mRNA expression levels of vascular endothelial growth factor(VEGF-A)and phosphatidylinositol 3-kinase(PI3K)in each group were determined by real-time fluorescence quantitative PCR(RT-PCR).Results Compared with the control group(SFPS 0 mg/L),the survival rate of H1299 cells decreased significantly after SFPS treatment(30,100,300,1000 mg/L)for fouty-eight hours(all<0.05),while the number of VM pipeline structures decreased significantly(all<0.05).The RT-PCR showed that different concentrations of SFPS could inhibit the mRNA expressions of VEGF-A and PI3K in H1299 lung cancer cells(all<0.05).Conclusions SFPS can reduce the survival rate of H1299 lung cancer cells and inhibit VM formation by downregulation of VEGF-A and PI3K ex-pression.

关 键 词：羊栖菜多糖 肺肿瘤 癌 血管生成拟态 血管内皮生长因子 胞内磷脂酰肌醇激酶 

分 类 号：R734.2[医药卫生—肿瘤]