枣HMGR基因家族鉴定及表达分析  

Identification and Expression Analysis of HMGR Gene Family in Jujube(Ziziphus jujuba)

作　　者：李娜 韩红艳[1] 宋宇琴[2] 冯新新 李六林[2] 李洁[3] 化延斌 LI Na;HAN Hong-Yan;SONG Yu-Qin;FENG Xin-Xin;LI Liu-Lin;LI Jie;HUA Yan-Bin(Department of Biological Sciences and Technology,Jinzhong University,Jinzhong 030619,China;College of Horticulture,Shanxi Agricultural University,Jinzhong 030801,China;College of Forestry,Shanxi Agricultural University,Jinzhong 030801,China;Jiexiu Agriculture and Rural Bureau,Jinzhong 032000,China)

机构地区：[1]晋中学院生物科学与技术系,晋中030619 [2]山西农业大学园艺学院,晋中030801 [3]山西农业大学林学院,晋中030801 [4]山西省介休市农业农村局,晋中032000

出　　处：《农业生物技术学报》2025年第4期743-754,共12页Journal of Agricultural Biotechnology

基　　金：山西省高等学校科技创新计划(2023L313);山西省基础研究计划(自由探索类)(202303021222261);博士科研启动(2020BQ43);晋中学院博士专项资金(JUD2022002)。

摘　　要：萜类化合物是枣(Ziziphus jujuba)发挥药用功能的主要物质,3-羟基-3-甲基戊二酰-CoA还原酶(3-hydroxy-3-methylglutaryl-CoA reductase,HMGR)对植物萜类化合物生物合成具有重要作用。为了深入研究枣HMGR基因功能,本研究利用隐马尔可夫模型(HMM)对枣HMGR基因家族进行鉴定,采用生物信息学分析软件分析ZjHMGR基因家族成员的理化特征、基因结构和进化关系,采用qRT-PCR分析HMGR基因表达模式。结果表明,枣基因组中有21个HMGR基因成员,根据基因在染色体上的位置将其命名为ZjHMGR1~ZjHMGR21。21个ZjHMGR基因位于10条固定染色体和2条未放置的基因组支架上。ZjHMGR基因的CDS长度为271~3 425 bp,编码88~1 122个氨基酸,且分子量为9 827.05~124 950.93 kD。将枣与拟南芥(Arabidopsis thaliana)、苹果(Malus pumila)、白梨(Pyrus bretschneideri)、烟草(Nicotiana tabacum)和西瓜(Citrullus lanatus)的HMGR家族成员蛋白全长序列进行比对并构建系统发育树发现,ZjHMGR1单独为一类,ZjHMGR11和ZjHMGR12、ZjHMGR5和ZjHMGR10被分别划分为一类,且这5个基因家族成员与拟南芥、苹果、白梨和西瓜HMGR基因存在共线性关系;ZjHMGR基因2.0 kb启动子区域存在与激素相关的顺式元件,其中11个基因有赤霉素、茉莉酸甲酯和脱落酸响应顺式元件,8个基因有生长素响应顺式元件,12个基因有水杨酸响应顺式元件。与幼果相比,成熟果实ZjHMGR基因表达量显著升高(P<0.05),且'临黄1号'和'木枣'果实ZjHMGR基因的表达量与叶绿素a、叶绿素b、总叶绿素和花青苷的含量显著负相关,而与类胡萝卜素含量正相关。ZjHMGR基因家族在果实成熟过程中发挥重要作用。本研究为枣萜类物质合成的遗传改良提供参考。Terpenoids are the main substances for the medicinal function of jujube(Ziziphus jujuba).3-hydroxy-3-methylglutaryl-CoA reductase(HMGR) plays an important role in the biosynthesis of plant terpenoids.In order to further study the function of jujube HMGR genes,this study used HMM to identify the jujube HMGR gene family.Bioinformatics analysis software was used to analyze the physicochemical characteristics,gene structure,and evolutionary relationship of ZjHMGR genes.qRT-PCR was used to analyze the expression pattern of HMGR genes.The results showed that there were 21 HMGR gene members in the jujube genome,which were named ZjHMGR1~ZjHMGR21 according to their positions on the chromosome.The 21 HMGR genes were located on 10 chromosomes and 2 unplaced genomic scaffolds.The open reading frame(ORF) length of the ZjHMGR genes was 271 to 3 425 bp,encoding 88 to 1 122 amino acids,and the molecular weight ranged from 9 827.05 to 124 950.93 kD.The phylogenetic analysis of jujube and arabidopsis(Arabidopsis thaliana),apple(Malus pumila),pear(Pyrus bretschneideri),tobacco(Nicotiana tabacum) and watermelon(Citrullus lanatus) showed that the ZjHMGR1 was a separate group,while the ZjHMGR11 and ZjHMGR12,ZjHMGR5 and ZjHMGR10 were classified as separate class.And these 5 gene family members had collinear relationships with arabidopsis,apple,pear and watermelon.There were hormone-related ciselements in the 2.0 kb promoter region of ZjHMGR gene,among which 11 candidate genes had gibberellin,methyl jasmonate and abscisic acid response cis-elements,8 candidate genes had auxin response cis-elements,and 12 candidate genes have salicylic acid response cis-elements.Compared with young fruits,the expression level of ZjHMGR gene in mature fruits was significantly increased.The expression levels of the ZjHMGR genes in 'Linhuang No.1' and 'Muzao' fruits were significantly negatively correlated with the contents of chlorophyll a,chlorophyll b,total chlorophyll and anthocyanins,but positively correlated with the contents of carotenoids.ZjHMGR pla

关 键 词：枣 3-羟基-3-甲基戊二酰-CoA还原酶(HMGR) 甲羟戊酸 类胡萝卜素 

分 类 号：S662.9[农业科学—果树学]