基于网络药理学和分子对接探究人参缓解肌少症的作用机制  

Exploring the Mechanism of Panax ginseng in Relieving Sarcopenia Based on Network Pharmacology and Molecular Docking

作　　者：张早伟 谢果 喻勤[2] 毛新亮 严建刚 汪旭霆 杜玉兰 刘文利 ZHANG Zaowei;XIE Guo;YU Qin;MAO Xinliang;YAN Jiangang;WANG Xuting;DU Yulan;LIU Wenli(College of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Perfect(Guangdong)Commodity Co.,Ltd.,Zhongshan 528400,China;University of Electronic Science and Technology of China,Zhongshan Institute,Zhongshan 528400,China)

机构地区：[1]广州中医药大学中药学院,广州510006 [2]完美(广东)日用品有限公司,中山528400 [3]电子科技大学中山学院,中山528400

出　　处：《食品与发酵科技》2025年第2期67-77,共11页Food and Fermentation Science & Technology

基　　金：国家自然科学基金项目(32101903)。

摘　　要：采用网络药理学和分子对接方法探究人参缓解肌少症作用机制。通过多种靶点数据库获得人参的潜在靶点,并通过多种疾病数据库获得肌少症疾病靶点。构建人参缓解肌少症的蛋白互作网络图。使用R语言进行GO和KEGG富集分析,并通过Autodock vina进行分子对接验证其机制。结果表明,人参的活性成分17个,预测可能作用的靶点578个;肌少症相关疾病靶点572个;人参与肌少症的共同靶点58个。通过对人参与肌少症的共同靶点进行GO和KEGG富集分析可以发现,人参可能通过对炎症反应的调节、对类固醇激素的反应、炎症反应的正向调节、细胞的增殖以及多细胞生物过程发挥缓解肌少症的作用。通过HIF-1、AGE-RAGE和TNF等信号通路发挥缓解肌少症的作用。通过蛋白互作网络图以及分子对接的分析结果可知,人参缓解肌少症的核心成分可能是山柰酚、人参皂苷Rh4、马卡因、去氧哈林通碱、花生四烯酸、吉九里香碱,人参作用的核心靶点为AKT1、TNF和BCL2,是缓解肌少症的潜在核心靶点。Network pharmacology and molecular docking technology were used to explore the mechanism of Panax ginseng in alleviating sarcopenia.Potential targets for Panax ginseng were obtained through several target databases,and relevant disease targets for sarcopenia were obtained through several disease databases.The protein interaction network diagram of Panax ginseng alleviating sarcopenia was constructed.GO and KEGG enrichment were analyzed using R language,and their mechanisms were validated through molecular docking using Autodock vina.The results indicated that there were 17 active ingredients in Panax ginseng and 578 potential active targets;572 disease targets related to sarcopenia;58 common targets between Panax ginseng and sarcopenia.Through GO and KEGG enrichment analysis of common targets for Panax ginseng and sarcopenia,it could be found that ginseng may play a role in alleviating sarcopenia through regulation of inflammatory response,response to steroid hormones,positive regulation of inflammatory response,cell proliferation,and multicellular biological processes.Sarcopenia was relieved through signaling pathways such as HIF-1,AGE-RAGE,and TNF.According to the analysis results of protein interaction network diagram and molecular docking,it can be concluded that the core components of Panax ginseng in alleviating sarcopenia may be kaempferol,ginsenoside Rh4,maackiain,deoxyharringtonin,arachidonic acid,and girinimbine.The core targets of Panax ginseng are AKT1,TNF,and BCL2,which are potential core targets for alleviating sarcopenia.

关 键 词：人参 肌少症 炎症 网络药理学 分子对接 

分 类 号：R285[医药卫生—中药学]