桑树内生菌的分离鉴定及功能菌筛选  

Identification and Functional Characterization of Endophytic Microbes from Mulberry Tree(Morus alba L.)

作　　者：李莎 杨加虎 王艳辉[1] 黄新球[1] 杨文[1] LI Sha;YANG Jiahu;WANG Yanhui;HUANG Xinqiu;YANG Wen(Sericultural and Apicultural Research Institute,Yunnan Academy of Agricultural Sciences,Mengzi 661101,China)

机构地区：[1]云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101

出　　处：《河南农业科学》2025年第3期77-86,共10页Journal of Henan Agricultural Sciences

基　　金：云南省农业联合基金面上项目(202301BD070001-223);云南省农业科学院蚕蜂所2022年度青年创新基金项目(QC2022004)。

摘　　要：采用微生物培养、目标基因序列扩增与同源性鉴定等方法对桑树(Morus alba L.)内生菌进行分离与鉴定,探讨云桑6号桑树根、茎、叶中的内生细菌和内生真菌的菌株种类、分布特性及菌株功能特性。结果表明,从桑树中共分离得到9属43株内生细菌及14属22株内生真菌,其中从根部分离得到21株细菌和11株真菌,从茎中分离得到13株细菌和5株真菌,从叶中分离得到9株细菌和6株真菌。桑树根、茎、叶中的内生菌分布差异大,内生细菌在桑树根与茎中共有白色假芽孢杆菌(Falsibacillus pallidus)、贝莱斯芽孢杆菌(Bacillus velezensis)、韩国假单胞菌(Pseudomonas koreensis),茎与叶中共有成团泛菌(Pantoea agglomerans)、枯草芽孢杆菌(Bacillus subtilis)、韩国假单胞菌(Pseudomonas koreensis),根、茎、叶中共有韩国假单胞菌(Pseudomonas koreensis);内生真菌在桑树根与叶中共有炭疽病菌(Colletotrichum siamense),茎与叶、根与茎中没有检测到共有真菌存在。进一步的功能菌筛选结果表明,从65株内生菌中分别得到8株固氮功能菌(7株细菌、1株真菌)、4株溶磷菌(2株细菌、2株真菌)和8株解钾功能菌(6株细菌、2株真菌)。综上,桑树内生细菌、内生真菌在根、茎、叶中具有多样性和分布差异性,其中有溶磷、固氮、解钾等功能菌存在。This study used methods such as microbial culture,target gene sequence amplification,and homology identification to isolate and identify endophytic microbes in Morus alba L.,and explored the strain types,distribution characteristics,and functional characteristics of endophytic bacteria and fungi in the roots,stems,and leaves of Yunsang No.6 mulberry.The results showed that a total of 43 endophytic bacteria belonging to 9 genera and 22 endophytic fungi belonging to 14 genera were isolated from mulberry trees.Among them,21 bacteria and 11 fungi were isolated from roots,13 bacteria and 5 fungi were isolated from stems,and 9 bacteria and 6 fungi were isolated from leaves.There were significant differences in the distribution of endophytic microbes in the roots,stems,and leaves of mulberry trees,there were Falsibacillus pallidus,Bacillus velezensis and Pseudomonas koreensis shared in the roots and stems of mulberry trees,Pantoea agglomerans,Bacillus subtilis and Pseudomonas koreensis shared in the stems and leaves,and Pseudomonas koreensis shared in the roots,stems,and leaves;Endophytic fungi Colletotrichum siamense was shared in mulberry roots and leaves,but no common fungi were found to exist in the stems and leaves,stems and roots.Further screening of functional microbes showed that 8 strains of nitrogen fixing microbes(7 bacteria,1 fungus),4 strains of phosphorus solubilizing microbes(2 bacteria,2 fungi),and 8 strains of potassium solubilizing microbes(6 bacteria,2 fungi)were obtained from 65endophytic microbes,respectively.In summary,endophytic bacteria and fungi in mulberry trees exhibit diversity and distribution differences in roots,stems,and leaves.Among endophytic bacteria and fungi in mulberry trees,there are functional strains that can dissolve phosphorus,nitrogen,and potassium.

关 键 词：桑树 内生真菌 内生细菌 分离鉴定 功能菌 

分 类 号：S888.7[农业科学—特种经济动物饲养]