荔枝壳中根皮苷的提取工艺优化及其抗氧化活性分析  

Optimization of the Extraction Process and Analysis of the Antioxidant Activity of Phlorizin from Litchi Shell

作　　者：孟弘茹 徐双园 林之灏 孙铭飞 刘文俊 戚南山 李娟 张晓爱 林栩慧 吕敏娜 蔡海明 宋勇乐 陈祥杰 朱易斌 张健騑 李颖鑫 廖申权 MENG Hongru;XU Shuangyuan;LIN Zhihao;SUN Mingfei;LIU Wenjun;QI Nanshan;LI Juan;ZHANG Xiaoai;LIN Xuhui;LV Minna;CAI Haiming;SONG Yongle;CHEN Xiangjie;ZHU Yibin;ZHANG Jianfei;LI Yingxin;LIAO Shenquan(College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou Guangdong 510225;Institute of Animal Health,Guangdong Academy of Agricultural Sciences/Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control/Key Laboratory of Livestock Disease Prevention of Guangdong Province,Guangzhou Guangdong 510640;Guangzhou University of Chinese Medicine,Guangzhou Guangdong 510006;Agro-biological Gene Research Center of Guangdong Academy of Agricultural Sciences,Guangzhou Guangdong 510640;Shenzhen Animal and Plant Disease Prevention and Control Center,Shenzhen Guangdong 518052)

机构地区：[1]仲恺农业工程学院动物科技学院,广东广州510225 [2]广东省农业科学院动物卫生研究所/农业农村部禽流感等家禽重大疫病防控重点实验室/广东省畜禽疫病防治研究重点实验室,广东广州510640 [3]广州中医药大学,广东广州510006 [4]广东省农业科学院农业生物基因研究中心,广东广州510640 [5]深圳市动植物疫病预防控制中心,广东深圳518055

出　　处：《广东畜牧兽医科技》2025年第2期1-7,共7页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省农业科学院协同创新中心项目(XTXM202202);科技创新战略专项资金(高水平农科院建设)(202122TD);广州市科技计划项目(2023B04J0137,2023A04J0789)。

摘　　要：为优化荔枝壳中根皮苷的提取工艺并分析根皮苷提取物的抗氧化活性,该研究通过优化料液比、溶剂浓度、提取温度、溶剂pH值和提取时间等单因素条件,以提高从荔枝壳中提取根皮苷的效率,并采用DPPH法和ABTS法分析所提取根皮苷的抗氧化活性。单因素分析试验结果表明,以1∶40料液比提取根皮苷得率为1.22%,以65%乙醇溶液提取根皮苷得率为1.20%,在提取温度为60℃条件下提取根皮苷得率为1.51%,以溶剂(pH 5)提取根皮苷得率为1.58%,当提取时间为60 min时根皮苷的得率为1.39%。优化所得提取工艺条件为:料液比1∶40,乙醇浓度65%,提取温度60℃,溶剂pH值5,提取时间60 min,在此条件下荔枝壳根皮苷提取率为1.16%-1.39%。检测提取物的抗氧化活性发现,根皮苷提取物的抗氧化能力(TEAC值)为0.34μg TE/μg,总抗氧化能力为(4.02±1.08)mmol/g,表明从荔枝壳中提取的根皮苷具有较好的抗氧化活性,为开发荔枝壳来源的抗氧化制剂提供理论依据。To optimize the extraction process of phlorizin from litchi pericarp and assess its antioxidant activity,we enhanced the extraction efficiency by optimizing several parameters,including the solid⁃liquid ratio,solvent concentration,extraction temperature,solvent pH,and extraction duration.The antioxidant activity of phlorizin was analyzed using the DPPH and ABTS radical scavenging assays.Single⁃factor experimental results indicated the following extraction rates:a solid⁃liquid ratio of 1∶40 yielded 1.22%phlorizin,a 65%ethanol solution resulted in 1.20%,a water bath temperature of 60℃produced 1.51%,and a solvent pH of 5 achieved 1.58%.After an extraction time of 60 min,the yield of phlorizin reached 1.39%.The optimized extraction conditions included a solidliquid ratio of 1∶40,65%ethanol concentration,60℃temperature,pH 5,and a 60minute extraction time.These conditions yielded the extraction rate of phlorizin from litchi shell ranged between 1.16%and 1.39%.The antioxidant activity of the extracts demonstrated that the antioxidant capacity(TEAC value)of the phlorizin extract was 0.34μg TE/μg,with a total antioxidant capacity of(4.02±1.08)mmol/g.These findings provide a scientific basis for developing antioxidant preparations derived from litchi shell.

关 键 词：荔枝壳 根皮苷 提取工艺 抗氧化活性 

分 类 号：S816.7[农业科学—饲料科学]