黄曲霉毒素B1对海鲈体成分、肝脏组织结构及毒素累积量的影响  

Effects of Alfatoxin B1 on Body Composition,Hepatic Histology and Toxin Accumulation in Lateolabrax Maculatus

作　　者：董芮绮 陈冰 黄文 赵红霞 朱喜锋 彭凯 DONG Ruiqi;CHEN Bing;HUANG Wen;ZHAO Hongxia;ZHU Xifeng;PENG Kai(Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangdong Key Laboratory of Animal Breeding and Nutrition,Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture in Rural Affairs,Guangzhou Guangdong 510640;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306;Guangzhou Fishtech Biotechnology Co.Ltd.,Guangzhou Guangdong 510640)

机构地区：[1]广东省农业科学院动物科学研究所/广东省畜禽育种与营养研究重点实验室/农业农村部华南动物营养与饲料重点实验室/广东省农业科学院,广东广州510640 [2]上海海洋大学水产与生命学院,上海201306 [3]广州飞禧特生物科技有限公司,广东广州510640

出　　处：《广东畜牧兽医科技》2025年第2期8-13,共6页Guangdong Journal of Animal and Veterinary Science

基　　金：国家自然科学基金(31902388);国家外国专家项目(G2022030077L);广东省自然科学基金面上项目(2022A1515010850);科技创新战略专项资金(高水平农科院建设)专项(R2021PY⁃QY001);科技创新战略专项资金(农业科研主力军建设)专项(R2023PY⁃QN003)。

摘　　要：该文旨在研究饲料中添加黄曲霉毒素B1(AFB1)对海鲈体成分、肝脏组织结构及毒素累积量的影响。选择初体重为(2.90±0.02)g的海鲈480尾,随机分为4组,每组3个重复,每个重复40尾鱼。在基础饲料G0(不添加AFB1)上分别添加0.1(G0.1)、0.5(G0.5)和1.0(G1.0)mg/kg的AFB1,配制4组试验饲料。采取饱食投喂,试验周期为56 d。结果表明,各组间全鱼的水分、粗蛋白、粗脂肪和灰分含量无显著差异(P>0.05)。AFB1可诱导海鲈肝脏组织损伤,且随着饲料中AFB1含量增加,肝细胞出现变性、坏死及脂肪空泡化的程度越严重。海鲈肝脏中AFB1的累积量高于肌肉,随着饲料中AFB1添加量的增加,肝脏中AFB1累积量显著升高(P<0.05)。综上所述,饲料中添加0.1-1.0 mg/kg AFB1可诱导肝脏组织损伤。AFB1在肝脏中的累积量高于肌肉,且与AFB1添加量呈正相关。This study was conducted to study the effects of dietary alfatoxin B1(AFB1)on body composition,liver histomorphology and toxin accumulation of Lateolabrax maculatus.A total of 480 L.maculatus with an initial body weight of(2.90±0.02)g were randomly divided into 4 groups with 3 replicates per group and 40 fish per replicate.Five isonitrogenous and isolipidic diets,that are the basal diet G0(without AFB1),G0.1(adding 0.1 mg/kg of AFB1),G0.5(adding 0.5 mg/kg of AFB1)and G1.0(adding 1.0 mg/kg of AFB1),were fed to apparent satiation during the 56⁃day feeding trial.Inclusion of 0.1-1.0 mg/kg AFB1 in diets did not affect body composition of fish(P>0.05),but induced liver tissue injury.With dietary AFB1 increasing,the degeneration and necrosis degree of hepatocytes and fat vacuolization became more serious.AFB1 accumulation in the liver of fish was higher than that in muscle,which was increased with dietary AFB1 increasing(P<0.05).In conclusion,dietary inclusion of 0.1-1.0 mg/kg AFB1 induced liver tissue injury of L.maculatus.AFB1 accumulation in liver of fish was higher than that in muscle,and was positively correlated with the supplemental level of AFB1 in diets.

关 键 词：黄曲霉毒素B1 海鲈 组织结构 毒素累积量 

分 类 号：S816.32[农业科学—饲料科学]