液相色谱串联质谱法测定中蜂蜜中58种药物残留  

Determination of 58 Drug Residues in Honey by Liquid Chromatography-Tandem Mass Spectrometry

作　　者：张航俊 阮鑫 商小金 王晓燕 刘靥 马骏骅 屈健 侯轩 ZHANG Hangjun;RUAN Xin;SHANG Xiaojin;WANG Xiaoyan;LIU Ye;MA Junhua;QU Jian;HOU Xuan(Zhejiang Provincial Center for Animal Disease Prevention and Control,Hangzhou 311119,China)

机构地区：[1]浙江省动物疫病预防控制中心,浙江杭州311119

出　　处：《现代食品》2025年第5期209-215,共7页Modern Food

摘　　要：本文建立高效液相色谱串联质谱法测定蜂蜜中58种药物残留的方法。试样经PBS-EDTA·2Na(pH=7)稀释,HLB柱净化,40℃氮气吹干后,用体积分数为10%的甲醇溶液溶解,液相色谱-串联质谱法测定。使用C_(18)(150 mm×3.0 mm,3μm)色谱柱分离,0.2%甲酸溶液+甲醇作为流动相进行梯度洗脱,通过多反应监测模式进行测定。结果表明,各目标化合物在1~200 ng·mL^(-1)线性关系良好,相关系数均大于0.99。各化合物的检出限在1~5μg·kg^(-1)、定量限在2~10μg·kg^(-1)。平均回收率在67.3%~117.2%,相对标准偏差均小于15%。该方法操作简便、结果准确、稳定性好,适用于测定中蜂蜜中58种药物残留。A method for the determination of 58 drug residues in medium honey by high performance liquid chromatography-tandem mass spectrometry was developed.The samples were diluted by PBS-EDTA·2Na(pH=7),purified by HLB column,dried by nitrogen at 40℃,dissolved in methanol solution with volume fraction of 10%,and determined by liquid chromatoc-tandem mass spectrometry.The separation was performed on a C_(18)(150 mm×3.0 mm,3μm)column with 0.2%formic acid solution+methanol as the mobile phase for gradient elution,and the determination was performed by multi-reaction monitoring mode.The results showed that the linear relationship between the target compounds was good in the range of 1 ng·mL^(-1)to 200 ng·mL^(-1),and the correlation coefficients were all greater than 0.99.The limits of detection of each compound was 1μg·kg^(-1)to 5μg·kg^(-1),and limits of quantitation was 2μg·kg^(-1)to 10μg·kg^(-1).The average recoveries ranged from 67.3%to 117.2%,and the relative standard deviations were all less than 15%.The method is simple,accurate and stable,and is suitable for the determination of 58 kinds of drug residues in medium honey.

关 键 词：多类药物残留 蜂蜜 液相色谱串联质谱法 

分 类 号：O657.63[理学—分析化学]