基于空间代谢组学揭示口腔鳞状细胞癌诊断标志物  

Revealing Metabolic Markers of Oral Squamous Cell Carcinoma Diagnosis Based on Spatial Metabolomics

作　　者：赵惠婷 韩伟[3] 罗观发 卢雯 付玉娟 谭潇 胡勤刚[3] 杨细虎 ZHAO Huiting;HAN Wei;LUO Guanfa;LU Wen;FU Yujuan;TAN Xiao;HU Qingang;YANG Xihu(School of Stomatology,Jinzhou Medical University,Jinzhou 121001,China;Department of Oral and Maxillofacial Surgery,Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China;Department of Oral and Maxillofacial Surgery,Nanjing Stomatological Hospital,Medical School of Nanjing University,Nanjing 210008,China.)

机构地区：[1]锦州医科大学口腔医学院,辽宁锦州121001 [2]江苏大学附属医院口腔颌面外科,江苏镇江212001 [3]南京大学医学院附属口腔医院口腔颌面外科,江苏南京210008

出　　处：《口腔医学研究》2025年第4期287-292,共6页Journal of Oral Science Research

基　　金：国家自然科学基金面上项目(编号:82173380);江苏省重点研发计划(编号:BE2022671);镇江市社会发展指导性科技计划项目(编号:FZ2024054)。

摘　　要：目的:通过解吸电喷雾电离质谱成像(desorption electrospray ionization-mass spectrometry imaging,DESI-MSI)研究口腔鳞状细胞癌(oral squamous cell carcinomas,OSCC)的潜在生物标志物和可能的代谢途径。方法:本研究纳入的10例OSCC病例均为在江苏大学附属医院口腔颌面外科就诊住院且接受手术治疗的原发病例。收集组织样本用于DESI-MSI检测,进一步采用多元及单变量统计分析筛选差异代谢物,然后用受试者工作特征曲线(receiver operating characteristic curve,ROC曲线)分析差异代谢物的诊断能力。最后,进行京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)代谢通路分析确定相关的代谢通路。结果:OSCC肿瘤组织与正常组织共鉴定出144种差异代谢物,大多数为脂肪酸。ROC曲线分析发现这144种代谢物曲线下的面积(area under the curve,AUC)值均大于0.7。KEGG代谢通路分析发现,与OSCC发生相关的通路为不饱和脂肪酸的生物合成以及脂肪酸的生物合成。结论:基于DESI-MSI筛选了具有诊断价值的差异代谢物,发现OSCC组织存在不饱和脂肪酸的生物合成以及脂肪酸的生物合成代谢紊乱,可能与OSCC的发生发展密切相关。Objective:To identify potential biomarkers and possible metabolic pathways of oral squamous cell carcinomas(OSCC)through desorption electrospray ionization mass spectrometry imaging(DESI-MSI).Methods:Ten cases of OSCC included in this study were all primary cases treated with surgery in the Department of Oral and Maxillofacial Surgery of Jiangsu University Affiliated Hospital.Tissue samples were collected for detection by DESI-MSI system,and differential metabolites were identified using multivariate and univariate statistical analysis.The diagnostic ability of differential metabolites was further analyzed using receiver operating characteristic curve(ROC curve)analysis.Finally,Kyoto Encyclopedia of Genes and Genomes(KEGG)metabolic pathway analysis was performed to determine the relevant metabolic pathways.Results:According to metabolic profile,a total of 144 metabolites were identified in tumor tissue and normal tissue,most of which were fatty acids.ROC curve analysis was further performed and it was found that area under the curve(AUC)of all 144 metabolites was greater than 0.7.The ion imaging of metabolites accurately distinguished tumor tissue from normal tissue.The differential metabolites between tumor tissue and normal tissue mainly participate in two metabolic pathways,namely the biosynthesis of unsaturated fatty acids and biosynthesis of fatty acids.Conclusion:Based on DESI-MSI,differential metabolites with diagnostic value were screened,and the metabolism dysregulation of biosynthesis of unsaturated fatty acids and fatty acids in OSCC tissues was found,which may be closely related to the occurrence and development of OSCC.

关 键 词：口腔鳞状细胞癌 解吸电喷雾电离质谱成像 空间代谢组学 脂质代谢 

分 类 号：R739.8[医药卫生—肿瘤]