基于转录组测序的薰衣草响应温度变化的光合作用分子机制  

Molecular Mechanisms of Photosynthesis Response to Temperature in Lavandula angustifolia Based on Transcriptome Sequencing

作　　者：李玲 曲嘉琪 时丕秀 付畅[1] 杨佳瑶 梁雨晨 苑泽宁[1] Li Ling;Qu Jiaqi;Shi Pixiu;Fu Chang;Yang Jiayao;Liang Yuchen;Yuan Zening(Key Laboratory of Biodiversity of Aquatic Organisms,College of Life Sciences and Technology,Harbin Normal University,Harbin,150025)

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江省水生生物多样性研究重点实验室,哈尔滨150025

出　　处：《分子植物育种》2025年第4期1073-1083,共11页Molecular Plant Breeding

基　　金：大学生创新创业训练计划项目创新训练国家级项目(201910231028);黑龙江省大学生创新创业训练计划项目创新训练省级重点项目(202010231024)共同资助。

摘　　要：本研究采用高通量转录组测序技术,以生长在哈尔滨地区的薰衣草(Lavandula angustifolia Mill.)叶片为材料,对不同温度(26.67℃,F;32.07℃,S;-0.76℃,T)下获得的基因进行功能分类注释,分析了薰衣草转录组的整体表达特征及光合作用通路中差异表达基因响应温度变化的调控机制。结果表明,薰衣草叶片转录组共有90205条unigenes和315378条transcript,GC含量为42.12%,N50长度为1535 bp,表明转录组组装完整性较高。薰衣草unigenes在生物过程中涉及的功能亚类最多,其中,细胞过程和代谢过程所占比例最高达50.28%。在生物过程中,T vs S、F vs S、T vs F时均为光合作用富集程度最高。CCA分析表明差异基因的表达受温度的显著影响(P<0.01)。其中,T vs S时,下调表达基因比例高于上调表达基因,但在光合作用通路中,PSⅡ中的PsbD呈上调表达,可促进PSⅡ的修复;同时,PSⅠ中的PsaA呈上调表达,可保护PSI复合物,有助于植物在低温下维持正常光合作用。在光合天线蛋白通路中,F vs S时,上调表达基因比例高于下调表达基因,捕光复合体Ⅱ叶绿素a/b结合蛋白基因Lhcb3、Lhcb4、Lhcb5上调表达,利于植物维持PSII-LHCⅡ超复合体的稳定性,发挥光保护作用。本研究结果可为薰衣草的分子遗传育种及在中国东北地区种植推广奠定理论基础。In this study,the genes obtained under the different temperatures(26.67℃,F;32.07℃,S;-0.76℃,T)were annotated for functional classification using leaves of Lavandula angustifolia Mill.grown in Harbin area via high-throughput transcriptome sequencing technology.The overall expression characteristics of transcriptome and the regulatory mechanisms of differentially expressed genes in the photosynthetic pathway in response to different temperature were analyzed.The results showed that the transcriptome of L.angustifolia had a total of 90205 unigenes and 315378 transcripts with a GC content of 42.12%and an N50 length of 1535 bp implying a high transcriptome assembly integrity.The unigenes of L.angustifolia were involved in the most functional subclasses in biological processes,among which the highest proportion of cellular processes and metabolic processes was 50.28%.Among the biological processes,the highest photosynthetic enrichment was observed in T vs S,F vs S,and T vs F.The CCA analysis showed that differentially expressed genes was significantly affected by temperature(P<0.01).As T vs S,the proportion of down-regulated expression genes was higher than that of up-regulated expression genes,but PsbD in PSII was up-regulated in the photosynthetic pathway to promote PSII repair;meanwhile,PsaA in PSI was up-regulated to protect the PSI complex and help plants maintain normal photosynthesis at low temperatures.As F vs S,the proportion of up-regulated expressed genes was higher than that of down-regulated expressed genes,the up-regulated expression of chlorophyll a/b binding protein genes Lhcb3,Lhcb4 and Lhcb5 in the light trapping complexII facilitated plants to maintain the stability of PSII-LHCII supercomplex and perform the function of photoprotection in the photosynthetic antenna protein pathway.The results could lay a theoretical foundation for the molecular genetic breeding of L.angustifolia and its planting and promotion in northeastern China.

关 键 词：薰衣草 转录组测序 光合作用基因 温度 

分 类 号：S685.12[农业科学—观赏园艺]