基于WGCNA分析系统性红斑狼疮的关键基因  

Analysis of key genes in systemic lupus erythematosus based on WGCNA

作　　者：张琳萄 陈春丽 崔道林 Zhang Lintao;Chen Chunli;Cui Daolin(Institute of Function and Morphology,Qujing Medical College,Qujing,Yunnan 655000,China)

机构地区：[1]曲靖医学高等专科学校机能与形态研究所,云南曲靖655000

出　　处：《齐齐哈尔医学院学报》2025年第7期607-612,共6页Journal of Qiqihar Medical University

基　　金：云南省教育厅科学研究基金项目(2023J1760)。

摘　　要：目的 基于加权基因共表达网络分析(WGCNA)方法,筛选与系统性红斑狼疮(SLE)相关的高风险致病基因及其潜在的生物学过程和信号通路,以为SLE的发生、发展及其发病机制提供理论依据。方法 从GEO数据库中下载GSE72326基因表达数据集,利用R语言的“WGCNA”包分析SLE患者和正常人血液中的基因共表达变化,并通过“ClusterProfiler”包对与SLE密切相关的基因模块进行功能富集分析。使用“LIMMA”包筛选差异表达基因(DEGs),将DEGs与WGCNA筛选出的共同致病基因进行结合分析,并导入STRING数据库进行蛋白-蛋白相互作用(PPI)网络分析。最终,通过Cytoscape软件筛选出关键基因(Hub基因)。结果 共筛选出91个差异表达基因,鉴定出1个与SLE密切相关的基因模块(黄色模块,Cor=0.67,P=8.3e-14),该模块主要涉及病毒防御、I型干扰素信号通路、细胞因子介导的信号通路等生物学过程。进一步筛选出15个Hub基因,包括:IFI44、IFI44L、IFIT1、IFIT2、IFIT3、RSAD2、IFIH1、ISG15、MX1、OAS1、OAS2、OAS3、IFI35、EIF2AK2、DDX58。结论 通过WGCNA生物信息学方法,筛选出了与SLE密切相关的15个关键基因,这为深入探索SLE的发病机制提供了理论支持,且这些基因可能成为未来SLE治疗的潜在靶点。Objective To identify high-risk pathogenic genes related to systemic lupus erythematosus(SLE),and its potential biological processes and signaling pathways using weighted gene co-expression network analysis(WGCNA),providing a theoretical basis for understanding the pathogenesis and progression of SLE.Methods The GSE72326 dataset was downloaded from the GEO database,and the“WGCNA”package in R language was used to analyze gene co-expression changes in the blood of SLE patients and normal individuals.Functional enrichment analysis of SLE-related gene modules was conducted using the“clusterProfiler”package.Differentially expressed genes(DEGs)were identified using the"LIMMA"package,and common pathogenic genes were extracted for protein-protein interaction(PPI)network analysis using the STRING database.Hub genes were subsequently identified using Cytoscape software.Results A total of 91 DEGs were identified,and one module highly correlated with SLE(yellow module,Cor=0.67,P=8.3e-14)was found,mainly involved in processes such as viral defense,type I interferon signaling pathway,and cytokine-mediated signaling.Fifteen hub genes were identified,including IFI44,IFI44L,IFIT1,IFIT2,IFIT3,RSAD2,IFIH1,ISG15,MX1,OAS1,OAS2,OAS3,IFI35,EIF2AK2,and DDX58.Conclusions 15 key genes highly related to SLE have been identified by the WGCNA bioinformatics approach,providing theoretical support for further exploration of the pathogenesis of SLE.These genes may serve as potential therapeutic targets for SLE treatment.

关 键 词：系统性红斑狼疮 生物信息学 关键基因 

分 类 号：R593.24[医药卫生—内科学]