新型纳米探针通过靶向MN受体抑制透明细胞肾细胞癌的应用研究  

Application of innovative nanoprobes for targeted inhibition of clear cell renal cell carcinoma via MN receptors

作　　者：祝汉文 闫溢烁 齐鑫 李聪[1] 王子琦 ZHU Hanwen;YAN Yishuo;QI Xin;LI Cong;WANG Ziqi(Department of Urology,Harbin Medical University Cancer Hospital,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学附属肿瘤医院泌尿外科,哈尔滨150081

出　　处：《医学新知》2025年第4期386-393,共8页New Medicine

基　　金：国家自然科学基金面上项目(82171999)。

摘　　要：目的评估新型纳米材料MN受体靶向抑制系统(MN receptor targeted inhibition system,MTIS)靶向并抑制透明细胞肾细胞癌(clear cell renal cell carcinoma,ccRCC)的作用。方法构建小分子多肽纳米探针MTIS,并将其作用于ccRCC细胞系786-O和Caki-1,使用激光扫描共聚焦显微镜(laser scanning confocal microscopy,CLSM)观察MTIS与MN的靶向效果;开展CCK-8实验和Transwell实验验证MTIS对ccRCC的抑制作用;使用Renca细胞系在小鼠皮下种瘤,运用MTIS尾静脉注射,观测其治疗效果。结果CLSM观察发现MTIS与肿瘤细胞呈现出良好的共定位效果;CCK-8实验和Transwell实验结果显示MTIS能够显著抑制肿瘤细胞的迁移和侵袭能力。此外,利用活体成像系统观察到经尾静脉注射的MTIS在小鼠肿瘤部位有效浓聚,接受MTIS治疗的小鼠肿瘤体积在治疗后14 d、21 d和28 d均显著小于对照组,差异具有统计学意义(P<0.05)。结论新型纳米材料MTIS可以精确靶向ccRCC细胞,并且抑制ccRCC细胞的迁移和侵袭,具有良好的抑制肿瘤效果。Objective To evaluate the role of novel nanomaterial MN receptor targeted inhibition system(MTIS)in targeting and inhibiting clear cell renal cell carcinoma(ccRCC).Methods The microprobe MTIS was designed and implemented for use with ccRCC cell lines 786-O and Caki-1.The targeting efficacy of both MTIS and MN was examined through laser scanning confocal microscopy(CLSM).CCK-8 and Transwell assays were conducted to confirm the inhibitory impact of MTIS on ccRCC.Additionally,the Renca cell line was utilized to induce subcutaneous tumors in mice,and MTIS was administered through tail vein injection to assess its therapeutic effect.Results The CLSM observations revealed that MTIS exhibited excellent colocalization with tumor cells.The results from CCK-8 and Transwell experiments further demonstrated that MTIS significantly inhibited the migration and invasion of tumor cells.Furthermore,the effective concentration of MTIS injected via tail vein at the tumor site in mice was observed using the in vivo imaging system(IVIS).Notably,the tumor volume in mice after 14,21 and 28 days treated with MTIS were markedly smaller compared to the control group(P<0.05).Conclusion The novel nanoprobe MTIS effectively targets ccRCC cells and demonstrates a significant inhibitory effect on their migration and invasion.

关 键 词：透明细胞肾细胞癌 肿瘤显影 纳米材料 靶向抑制 MN受体 

分 类 号：R737.11[医药卫生—肿瘤] R318.08[医药卫生—临床医学]