ACAA1在肝细胞癌中的表达及临床意义  

Expression and clinical significance of ACAA1 in hepatocellular carcinoma

作　　者：梁紫渊 罗文奇 莫颖禧 周晓莹 LIANG Ziyuan;LUO Wenqi;MO Yingxi;ZHOU Xiaoying(Institute of Life Sciences,Guangxi Medical University,Nanning 530021,China;High Incidence Tumor Prevention and Control Key Laboratory of the Ministry of Education,Guangxi Medical University,Nanning 530021,China;Pathology Department,Guangxi Medical University Cancer Hospital,Nanning 530021,China;Research Department,Guangxi Medical University Cancer Hospital,Nanning 530021,China)

机构地区：[1]广西医科大学生命科学研究院,南宁530021 [2]广西医科大学高发肿瘤防治教育部重点实验室,南宁530021 [3]广西医科大学附属肿瘤医院病理科,南宁530021 [4]广西医科大学附属肿瘤医院研究部,南宁530021

出　　处：《广西医科大学学报》2025年第2期268-276,共9页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.32260170)。

摘　　要：目的:探讨ACAA1在肝细胞癌(HCC)中的异常表达和临床意义。方法:分别通过TCGA数据库分析HCC组织和正常肝组织的转录和蛋白表达水平,比较ACAA1的表达水平及其与临床特征之间的相关性。使用受试者工作特征曲线(ROC曲线)评估ACAA1的HCC诊断效能。利用细胞增殖实验和迁移实验分析ACAA1对HCC的影响。结果:与正常对照组相比,ACAA1的mRNA和蛋白表达水平显著降低,其表达水平与患者年龄呈负相关关系(P<0.05);与未患肝硬化的HCC患者相比,在肝硬化患者中表达显著降低(P<0.01)。ACAA1 mRNA的曲线下面积(AUC)为0.936(P<0.001),灵敏度为90%、特异度为86.5%。过表达ACAA1抑制HCC细胞的增殖和迁移能力(P<0.001)。此外,ACAA1与多种免疫细胞浸润呈负相关关系,过表达ACAA1显著提升HCC细胞CD276的转录水平(P<0.001)。结论:ACAA1在HCC中扮演肿瘤抑制基因的角色,其可能作为HCC的诊断和预后标志物,并参与肿瘤免疫微环境的调控。Objective:To investigate the abnormal expression and clinical significance of ACAA1 in hepatocellular carcinoma(HCC).Methods:The transcriptional and protein expression levels of HCC tissues and normal liver tissues were analyzed by the TCGA database,and the expression levels of ACAA1 and their correlation with clinical characteristics were compared.The diagnostic potential of ACAA1 for HCC was evaluated using the receiver operating characteristic(ROC)curves.The effects of ACAA1 on HCC were analyzed through cell proliferation and migration assays.Results:Compared with the normal controls,both mRNA and protein expression levels of ACAA1 were significantly decreased.Their expression levels were negatively correlated with the age of the patients(P<0.05)and significantly reduced in the cirrhotic patients compared with the non-cirrhotic HCC patients(P<0.01).The area under the curve(AUC)for ACAA1 mRNA was 0.936(P<0.001),with a sensitivity of 90%and specificity of 86.5%.Overexpression of ACAA1 inhibited the proliferation and migration of HCC cells(P<0.001).Furthermore,ACAA1 expression was negatively correlated with the infiltration of various immune cells,and overexpression of ACAA1 significantly increased the transcriptional level of CD276 in HCC cells(P<0.001).Conclusion:ACAA1 acts as a tumor suppressor gene in HCC and may serve as a diagnostic and prognostic biomarker for HCC,as well as participate in the regulation of the tumor immune microenvironment.

关 键 词：肝细胞癌 生物标志物 免疫细胞浸润 预后 

分 类 号：R735.7[医药卫生—肿瘤]