不同产地肉豆蔻药材质量评价研究  

Study on quality evaluation of nutmeg from different origin

作　　者：王玥璇 朱小玲 王慧 向广军 孙国园 WANG Yuexuan;ZHU Xiaoling;WANG Hui;XIANG Guangjun;SUN Guoyuan(College of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China;Engineering Technology Research Center of Pharmacodynamic Substance and Quality Control of Mongolian Medicine in Inner Mongolia,Key Laboratory of Pharmacodynamic Substances of Inner Mongolia,Key Laboratory of Modernization of Traditional Prescriptions of Chinese and Mongolian Medicine in High Education Institutions of Inner mongolia,Hohhot 010110,China;Department of Pharmacy,Inner Mongolia International Mongolian Hospital,Hohhot 010065,China)

机构地区：[1]内蒙古医科大学药学院,呼和浩特010110 [2]内蒙古自治区蒙药药效物质与质量控制工程技术研究中心,内蒙古自治区药效物质研究重点实验室,中蒙药传统方剂现代化研究自治区高等学校重点实验室,呼和浩特010110 [3]内蒙古自治区国际蒙医医院药学部,呼和浩特010065

出　　处：《中华中医药杂志》2025年第3期1441-1447,共7页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82260838);内蒙古自治区科技计划项目(No.2019GG128);内蒙古自治区科技成果转化项目(No.2020GG0117);内蒙古自治区高等学校青年科技人才发展计划(No.NMGIR2226)。

摘　　要：目的:建立同时测定肉豆蔻药材中9种成分[OdoratisolA、5-烯丙基-1,2,3-三甲氧基苯、甲基丁香酚、α-细辛脑、(-)-Citronellol、肉豆蔻醚、去氢二异丁香酚、4-Isopropyltoluene、利卡灵]含量的高效液相色谱(HPLC)法,并利用多元统计分析方法评价不同产地肉豆蔻药材质量。方法:采用HPLC法,色谱柱为依利特Hypersil ODS(C_(18)),柱温为30℃;流动相为乙腈-水溶液,梯度洗脱;流速为0.6mL/min;检测波长为204nm。结果:9种成分线性关系良好;方法学考察符合要求,不同批次肉豆蔻9种成分的含量在0.0001%~0.7962%。结论:通过多成分含量测定,结合多元统计分析方法,可有效的评价不同产地肉豆蔻的质量,为控制肉豆蔻整体质量提供科学依据。Objective:To establish an high performance liquid chromatography(HPLC)method to determine the contents of nine components Odoratisol A,5-neneneba Allyll,2,3-trimethoxybenzene,methyleugenol,alpha-Asarone,(-)-Citronellol,Myristicin,Dehydrodisoeugenol,4-Isopropyltoluene and Licarinj from nutmeg,and to evaluate the quality of nutmeg medicinal materials from different regions by multiple statistical analysis methods.Methods:HPLC analysis was performed by an Elite C_(18) column at temperature 30℃with gradient elution(acetonitrile and aqueous solution)at a flow rate of 0.6 mL/min.Detection wavelength is 204 nm.Results:Nine components have good linear relationships.The methodological examination meets the requirements,and the content of 9 components in different batches of nutmeg ranges from 0.0001%to 0.7962%.Conclusion:The quality of nutmeg from different producing areas can be evaluated effectively by multi-component content determination combined with multivariate statistical analysis,which provides scientific basis for controlling the whole quality of nutmeg.

关 键 词：高效液相色谱 肉豆蔻 质量评价 肉豆蔻醚 去氢二异丁香酚 

分 类 号：R284.1[医药卫生—中药学]