碳量子点荧光法测定食品营养强化剂葡萄糖酸亚铁的含量  

Determination of food nutrition fortifier ferrous gluconate by carbon quantum dot fluorescence

作　　者：江书庆[1] 信建豪[1] JIANG Shu-qing;XIN Jian-hao(School of Medicine,Huanghe S&T University,Zhengzhou 450063)

机构地区：[1]黄河科技学院医学院,河南郑州450063

出　　处：《化学研究与应用》2025年第4期1034-1038,共5页Chemical Research and Application

基　　金：河南省研究生联合培养基地项目(YJS2024JD57)资助;河南省医学教育研究项目(WILX2023141)资助;郑州市医疗卫生领域科技创新指导计划项目(2024YLZDJH021)资助;郑州市基础研究与应用基础研究项目(ZZSZX202424)资助;郑州市基础研究与应用基础研究项目(ZZSZX202420)资助。

摘　　要：采用水热反应法制备碳量子点溶液,以此作为荧光探针,建立了一种Fe^(3+)的检测方法,并用来测定食品营养强化剂葡萄糖酸亚铁的含量。结果表明,以三羟甲基氨基甲烷和甘氨酸为原料,制备得到的碳量子点溶液荧光效率为27.54%。Fe^(3+)溶液在反应温度20℃、pH 1.00、作用时间4min时对碳量子点溶液的荧光猝灭效果最佳,浓度在1.00×10^(-5)~7.50×10^(-4)mol·L^(-1)范围内,与荧光值ΔF呈现良好的线性关系(R=0.9982),用于测定食品营养强化剂葡萄糖酸亚铁,结果为98.4%,加标回收率在96.08%~102.4%范围内,检出限为6.72μmol·L^(-1)。该方法可实时、实地快速测定葡萄糖酸亚铁的含量。Preparation of carbon quantum dot(CQD)solution via a hydrothermal reaction method has been employed as a fluorescent probe to establish a detection method for Fe^(3+)ions,which was then used to determine the content of ferrous gluconate,a food nutritional fortifier.The results indicate that the CQD solution,synthesized from tris(hydroxymethyl)aminomethane and glycine,exhibited a fluorescence efficiency of 27.54%.The optimal fluorescence quenching effect of Fe^(3+)solution on the CQD solution was achieved at a reaction temperature of 20℃,pH 1.00,and an action time of 4 minutes.Within the concentration range of 1.00×10^(-5)to 7.50×10^(-4)mol·L^(-1),a good linear relationship(R=0.9982)with the fluorescence changeΔF was observed.When applied to the determination of ferrous gluconate in food nutritional fortifiers,the average result is 98.4%,with a spiked recovery rate ranging from96.08%to 102.4%,and a detection limit of 6.72μmol·L^(-1).This method allows for rapid,on-site determination of ferrous gluconate content in real-time.

关 键 词：碳量子点 葡萄糖酸亚铁 荧光探针 测定方法 

分 类 号：O652.1[理学—分析化学]