β-烟酰胺单核苷酸、牛磺酸顺势给药和联合给药对大鼠实验性矽肺治疗效果  

Homeopathic dosing and combined dosing ofβ-nicotinamide mononucleotide and taurine on experimental silicosis in rats

作　　者：甘永金[1] 陈瑞泽 凌健安[1] 黄艳妮 梁丹玉[1] GAN Yongjin;CHEN Ruize;LING Jian’an;HUANG Yanni;LIANG Danyu(Institute of Occupational Health and Toxicity Evaluation,Guangxi Zhuang Autonomous Region Research Academy of Occupational Disease Prevention and Control,Nanning,Guangxi 530021,China)

机构地区：[1]广西壮族自治区职业病防治研究院职业卫生与毒性评价所,广西南宁530021

出　　处：《环境与职业医学》2025年第2期218-223,共6页Journal of Environmental and Occupational Medicine

基　　金：广西壮族自治区卫生健康委自筹经费科研课题研究项目(Z-A20230409)。

摘　　要：[背景]尘肺病是一种不可完全治愈的慢性炎症疾病。因此,控制肺部炎症以及延缓肌体的衰老是治疗尘肺病的关键之一。最近二十年,研究结果发现许多小分子药物对于增强心肺功能具有不错的保健和治疗效果。[目的]探讨顺势给药和联合给药β-烟酰胺单核苷酸和牛磺酸对大鼠实验性矽肺治疗效果。[方法]72只无特定病原体级SD大鼠,随机分为4组(每组18只):阴性对照组(超纯水,不染尘)、阳性对照组、顺势给药治疗组、联合给药治疗组。采用一次性非暴露式气管注入法向大鼠气管内注入1 mL石英粉尘混悬液(50 mg·mL^(-1)),建立大鼠矽肺模型。大鼠采用灌胃给药,染尘后第4天开始对顺势给药治疗组大鼠上午给予0.03 g·mL^(-1)牛磺酸溶液,下午给予0.03 g·mL^(-1)β-烟酰胺单核苷酸溶液;联合给药治疗组大鼠分早晚2次给予(0.015 g·mL^(-1)β-烟酰胺单核苷酸+0.015 g·mL^(-1)牛磺酸)的混合溶液;给予阴性对照组、阳性对照组早晚等量超纯水,4组大鼠均每周给药5 d,共给药6周。给药6周后处死大鼠,检测肺羟脯氨酸含量、全肺游离二氧化硅含量、肺灌洗液中细胞计数及分类、全肺干湿重、脏器系数,观察肺组织病理学变化。[结果]与阳性对照组比较,顺势给药治疗组、联合给药治疗组的肺羟脯氨酸含量、肺脏脏器系数、全肺湿重、干重、细胞总数、中性粒细胞率、全肺游离二氧化硅含量降低,差异均有统计学意义(P<0.05);与阴性对照组比较,顺势给药治疗组、联合给药治疗组的肺羟脯氨酸含量、肺脏脏器系数、细胞总数、中性粒细胞率、全肺游离二氧化硅含量升高,联合给药治疗组的全肺干重升高,差异均有统计学意义(P<0.05);大鼠肺组织病理结果显示,阳性对照组肺组织内形成境界清楚的圆形或椭圆形的结节,为吞噬性的细胞性结节,部分区域的肺泡结构尚存在,顺势给药治疗组、联合�[Background]Pneumoconiosis is a chronic inflammatory disease that cannot be completely cured.Therefore,how to control lung inflammation and delay of the body aging is one of the keys to treating pneumoconiosis.The studies in past two decades suggested that many small molecule drugs are able to enhance cardiopulmonary function.[Objective]To explore the effects of homeopathic dosing and combined dosing ofβ-nicotinamide mononucleotide and taurine on experimental silicosis in rats.[Methods]Seventy-two SD specific pathogen-free rats were randomized into 4 groups(18 mice in each group):negative control group(ultrapure water,without dust),positive control group,homeopathic treatment group,co-administered treatment group.One mL of quartz dust suspension was injected into the rat trachea by disposable non-exposed tracheal injection method(50 mg·mL^(−1))to establish a rat silicosis model.Rats were administered by gavage since the 4th day after dust exposure.The homeopathic treatment group rats received taurine solution(0.03 g·mL^(−1))in the morning andβ-nicotinamide mononucleotide(0.03 g·mL−1)in the afternoon;the co-administered treatment group rats received a mixed solution(0.015 g·mL^(−1)β-nicotinamide mononucleotide+0.015 g·mL^(−1) taurine)twice,in the morning and afternoon respectively.The positive and negative control groups received equivalent of ultrapure water in the morning and afternoon.All groups of rats were administered 5 d a week for a total of 6 weeks.The rats were neutralized after 6 weeks of administration.Organ coefficient,lung hydroxyproline content,whole lung dry and wet weights,whole lung free silica content,and cell count and classification in lung lavage fluid were measured and calculated,and lung histopathological changes in lung samples were observed.[Results]Compared with the positive control group,the whole lung wet weight,whole lung dry weight,total cell count,neutrophil rate,lung organ coefficient,lung hydroxyproline content,and whole lung free silica content were reduce in th

关 键 词：β-烟酰胺单核苷酸 牛磺酸 顺势给药 联合给药 染尘大鼠 游离二氧化硅 

分 类 号：R11[医药卫生—公共卫生与预防医学]