肠道菌群丰度及血清sFas、8-OHdG对重度阿尔茨海默病的预测价值  

Predictive value of intestinal flora abundance and serum sFas,8-OHdG in severe Alzheimer′s disease

作　　者：王鹏飞 李艳雪 王禹 孙安龙 WANG Pengfei;LI Yanxue;WANG Yu;SUN Anlong(Department of Neurology,Hebei Yanda Hospital,Langfang,Hebei 065401,China;Department of Neurology,Anxin County Hospital,Baoding,Hebei 071699,China;Department of Neurology,the Fourth People's Hospital of Langfang,Langfang,Hebei 065700,China)

机构地区：[1]河北燕达医院神经内科,河北廊坊065401 [2]河北省保定市安新县医院神经内科,河北保定071699 [3]河北省廊坊市第四人民医院神经内科,河北廊坊065700

出　　处：《检验医学与临床》2025年第8期1014-1018,1023,共6页Laboratory Medicine and Clinic

基　　金：河北省医学科学研究课题计划项目(20232047)。

摘　　要：目的 探讨肠道菌群丰度及血清可溶性凋亡相关因子(sFas)、8-羟基脱氧鸟苷(8-OHdG)对重度阿尔茨海默病(AD)的预测价值。方法 选取2022年5月至2024年4月河北燕达医院收治的102例AD患者作为AD组,另选取同期在河北燕达医院体检的114例健康志愿者作为对照组。根据临床痴呆评定量表(CDR)评分将AD患者分为轻中度组(CDR评分为1~2分)和重度组(CDR评分为3分)。采用高通量测序检测AD患者肠道菌群丰度,酶联免疫吸附试验检测血清sFas、8-OHdG、白细胞介素(IL)-10和IL-1β水平。采用Pearson相关分析血清sFas、8-OHdG水平与AD患者肠道菌群丰度的相关性。绘制受试者工作特征(ROC)曲线分析血清sFas、8-OHdG对重度AD的预测价值。结果 AD组血清sFas、8-OHdG水平高于对照组,差异均有统计学意义(P<0.05)。轻中度组有75例患者,重度组有27例患者。重度组简易智能精神状态量表评分低于轻中度组,差异有统计学意义(P<0.05)。重度组血清sFas、8-OHdG和IL-1β水平高于轻中度组,血清IL-10水平低于轻中度组,差异均有统计学意义(P<0.05)。重度组大肠杆菌属、葡萄球菌属菌群丰度高于轻中度组,双歧杆菌属和乳酸杆菌属菌群丰度低于轻中度组,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,AD患者血清sFas、8-OHdG水平与双歧杆菌属和乳酸杆菌属菌群丰度呈负相关(P<0.05),与葡萄球菌属菌群丰度呈正相关(P<0.05)。ROC曲线分析结果显示,血清sFas、8-OHdG联合预测重度AD的曲线下面积(AUC)为0.870,大于血清sFas单独预测的AUC(Z=2.011,P=0.044),但与血清8-OHdG单独预测的AUC比较,差异无统计学意义(Z=1.878,P=0.060)。结论 AD患者血清sFas、8-OHdG水平升高,且与患者肠道菌群丰度有关,对其严重程度具有一定预测价值。Objective To investigate the predictive value of intestinal flora abundance and serum soluble apoptosis related factors(sFas)and 8-hydroxydeoxyguanosine(8-OHdG)in severe Alzheimer′s disease(AD).Methods A total of 102 AD patients admitted to Hebei Yanda Hospital from May 2022 to April 2024 were selected as AD group,and 114 healthy volunteers who underwent physical examination in Hebei Yanda Hospital during the same period were selected as control group.According to the Clinical Dementia Rating Scale(CDR)score,they were divided into mild to moderate group(CDR score was 1-2 points)and severe group(CDR score was 3 points).High throughput sequencing was used to detect the abundance of intestinal flora in AD patients,and serum sFas,8-OHdG,interleukin(IL)-10 and IL-1βlevels were detected by enzyme-linked immunosorbent assay.Pearson correlation was used to analyze the correlation between serum sFas,8-OHdG levels and intestinal flora abundance in AD patients.Receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of serum sFas and 8-OHdG in severe AD.Results Serum sFas and 8-OHdG levels in AD group were higher than those in control group,and the differences were statistically significant(P<0.05).There were 75 patients in the mild to moderate group and 27 patients in the severe group.The Simple Intelligent Mental Transformation Quantitative Change Scale score of the severe group was lower than that of the mild to moderate group,with statistical significance(P<0.05).Serum sFas,8-OHdG and IL-1βlevels in the severe group were higher than those in the mild to moderate group,and serum IL-10 level in the severe group was lower than that in the mild to moderate group,with statistical significance(P<0.05).The abundance of Escherichia coli and Staphylococcus bacteria in the severe group was higher than that in the mild to moderate group,while the abundance of Bifidobacterium and Lactobacillus bacteria was lower than that in the mild to moderate group,with statistical significance(P<0.05).Pearson co

关 键 词：阿尔茨海默病 肠道菌群 可溶性凋亡相关因子 8-羟基脱氧鸟苷 预测价值 

分 类 号：R742[医药卫生—神经病学与精神病学] R749.16[医药卫生—临床医学]