乳腺癌组织miR-216b-5p、miR-589-5p与肿瘤恶性增殖基因表达和预后的关系  

Relationship between miR-216b-5p,miR-589-5p and tumor malignant proliferation gene expression and prognosis in breast cancer tissues

作　　者：何英 石先伟[2] 张雪琳 刘臻臻 朱克鹏 尹均明 杜果城 HE Ying;SHI Xianwei;ZHANG Xuelin;LIU Zhenzhen;ZHU Kepeng;YIN Junming;DU Guocheng(Department of Breast and Thyroid Vascular Surgery,Nanchong Central Hospital,Nanchong,Sichuan 637000,China;the Second Clinical Medical School of North Sichuan Medical University,Nanchong,Sichuan 63700,China)

机构地区：[1]南充市中心医院乳腺甲状腺血管外科,四川南充637000 [2]川北医学院第二临床医学院,四川南充637000

出　　处：《检验医学与临床》2025年第8期1116-1120,1126,共6页Laboratory Medicine and Clinic

基　　金：四川省南充市2022年市校科技战略合作专项(22SXQT0195)。

摘　　要：目的 探讨乳腺癌组织miR-216b-5p、miR-589-5p与肿瘤恶性增殖基因表达和预后的关系。方法 选取2017年8月至2020年8月南充市中心医院收治的490例乳腺癌患者作为研究对象。采用实时荧光定量聚合酶链反应检测癌组织与癌旁组织中miR-216b-5p、miR-589-5p及恶性增殖基因[Yes关联蛋白1(YAP1)、PIWIL样蛋白2(Piwil2)、zeste基因增强子同源物2(EZH2)]水平。随访3年,根据总生存情况将患者分为生存组和死亡组。采用Pearson相关分析乳腺癌组织miR-216b-5p、miR-589-5p水平与恶性增殖基因水平的相关性。采用Log-Rankχ2和Kaplan-Meier生存曲线分析乳腺癌患者3年生存情况。采用多因素Cox回归分析乳腺癌患者死亡的影响因素。结果 乳腺癌组织中miR-216b-5p、miR-589-5p水平低于癌旁组织,YAP1、Piwil2、EZH2信使RNA(mRNA)水平高于癌旁组织,差异均有统计学意义(P<0.05)。死亡组淋巴结转移、TNM分期为Ⅲ期、miR-216b-5p低表达、miR-589-5p低表达患者的比例高于生存组,差异均有统计学意义(P<0.05)。Kaplan-Meier生存曲线分析结果显示,miR-216b-5p低表达、miR-589-5p低表达、淋巴结转移和TNM分期为Ⅲ期乳腺癌患者生存曲线分别低于miR-216b-5p高表达、miR-589-5p高表达、无淋巴结转移和TNM分期为Ⅰ+Ⅱ期患者生存曲线(P<0.05)。多因素Cox回归分析结果显示,淋巴结转移、TNM分期为Ⅲ期、miR-216b-5p低表达、miR-589-5p低表达均是乳腺癌患者死亡的危险因素(P<0.05)。结论 与癌旁组织相比,乳腺癌组织miR-216b-5p、miR-589-5p水平降低,且与恶性增殖基因高表达、TNM分期增高、淋巴结转移有关。Objective To investigate the relationship between miR-216b-5p,miR-589-5p and tumor malignant proliferation gene expression and prognosis in breast cancer tissues.Methods A total of 490 breast cancer patients who were admitted to Nanchong Central Hospital from August 2017 to August 2020 were selected as study objects.MiR-216b-5p,miR-589-5p and malignant proliferating genes[Yes associated protein 1(YAP1),PIwiL-like protein 2(Piwil2)and zeste gene potentiator homology 2(EZH2)]levles in cancer tissue and paracancer tissue were detected by real-time fluorescence quantitative polymerase chain reaction.Patients were followed up for 3 years,and they were divided into survival group and death group according to overall survival.Pearson correlation was used to analyze the correlation between the levels of miR-216b-5p,miR-589-5p and the levels of malignant proliferation genes in breast cancer tissues.The 3 years survival of breast cancer patients was analyzed by Log-Rankχ2 and Kaplan-Meier survival curves.Multivariate Cox regression was used to analyze the influencing factors of death in breast cancer patients.Results MiR-216b-5p and miR-589-5p in breast cancer tissues were lower than those in paracancer tissues,while the messenger RNA(mRNA)levels of YAP1,Piwil2 and EZH2 were higher than those in paracancer tissues,with statistical significance(P<0.05).The proportions of patients with lymph node metastasis,TNM stageⅢ,miR-216b-5p low expression and miR-589-5p low expression in death group were higher than those in survival group,with statistical significance(P<0.05).Kaplan-Meier survival curve analysis results show that the survival curves of patients with miR-216b-5p low expression,miR-589-5p low expression,lymph node metastasis and TNM stageⅢwere lower than those of patients with miR-216b-5p high expression,miR-589-5p high expression,no lymph node metastasis and TNM stageⅠ+Ⅱrespectively(P<0.05).Multivariate Cox regression analysis showed that lymph node metastasis,TNM stageⅢ,miR-216b-5p low expression and miR-58

关 键 词：乳腺癌 miR-216b-5p miR-589-5p 恶性增殖基因 病理特征 预后 

分 类 号：R737.9[医药卫生—肿瘤] R446.8[医药卫生—临床医学]