TGF-β1对EGFR突变ⅢC-IV期NSCLC患者血清细胞因子水平的影响  

Effects of TGF-β1 on Serum Cytokines Levels in Stage ⅢC-IV Non-small Cell Lung Cancer Patients with EGFR Mutation

作　　者：王淑文[1] 徐泽惠 陈文换[1] 高建芝 WANG Shuwen;XU Zehui;CHEN Wenhuan;GAO Jianzhi(Hebei Province Zhuozhou Hospital,Hebei Zhuozhou 072750,China;Haiyuan College,Kunming Medical University,Yunnan Kunming 650106,China;Hebei Yizhou Cancer Hospital,Hebei Zhuozhou 072750,China)

机构地区：[1]河北省涿州市医院,河北涿州072750 [2]昆明医科大学海源学院,云南昆明650106 [3]河北一洲肿瘤医院,河北保定072750

出　　处：《中国医药导刊》2025年第2期187-190,共4页Chinese Journal of Medicinal Guide

基　　金：保定市科技局自然科学课题(2141ZF173)。

摘　　要：目的:探讨转化生长因子β(TGF-β)对表皮生长因子受体(EGFR)突变ⅢC-IV期非小细胞肺癌(NSCLC)患者血清细胞因子白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)及肿瘤坏死因子-α(TNF-α)的影响。方法:选取2018年5月至2022年1月于我院行基因检测的117例ⅢC-IV期NSCLC患者作为研究对象,分为EGFR突变组(n=50)和EGFR未突变组(n=67),应用免疫组织化学(IHC)染色观察TGF-β1的蛋白表达情况,ELISA法检测血清细胞因子IL-2、IL-6、IL-10及TNF-α的水平,分析TGF-β1对EGFR突变ⅢC-IV期NSCLC患者血清细胞因子IL-2、IL-6、IL-10及TNF-α的影响。结果:EGFR突变组患者TGF-β1蛋白表达水平高于未突变组(P<0.05)。与EGFR未突变组比较,突变组患者血清细胞因子IL-2在TGF-β1高表达时水平降低(P<0.05),IL-6、IL-10及TNF-α在TGF-β1高表达时水平均增高(P<0.05)。结论:EGFR突变ⅢC-IV期NSCLC患者TGF-β1表达上调可影响血清细胞因子IL-2、IL-6、IL-10及TNF-α的水平,改变肿瘤免疫微环境,加强肿瘤免疫逃逸。Objective:To investigate the effects of transforming growth factor-β(TGF-β1)on serum cytokines interleukins-2(IL-2),interleukins-6(IL-6),interleukins-10(IL-10),and tumor necrosis factor-α(TNF-α)in stageⅢC-IV non-small cell lung cancer(NSCLC)patients with epidermal growth factor receptor(EGFR)mutation.Methods:117 patients with stageⅢC-IV NSCLC who had gene detection in our hospital from May 2018 to January 2022 were selected as the research objects,and were divided into the EGFR mutant group(n=50)and the EGFR non-mutant group(n=67).The protein expression of TGF-β1 was observed by immunohistochemi-cal staining.The levels of serum cytokines IL-2,IL-6,IL-10 and TNF-αwere detected by enzyme linked immunosorbent assay(ELISA).The effects of TGF-β1 on serum cytokines IL-2,IL-6,IL-10 and TNF-αin stageⅢC-IV NSCLC patients with EGFR muta-tion were analyzed.Results:The expression of TGF-β1 in the EGFR mutant group was higher than that in the non-mutant group(P<0.05).Compared with EGFR non-mutant grroup,the level of IL-2 decreased when the expression of TGF-β1 in the mutant group was high(P<0.05),while the levels of IL-6,IL-10 and TNF-αincreased when the expression of TGF-β1 was high(P<0.05).Conclusion:The up-regulation of TGF-β1 expression in stageⅢC-IV NSCLC patients with EGFR mutation can affect the levels of serum cytokines 2025 Volume 27 No.2(Serial No.256)IL-2,IL-6,IL-10 and TNF-α,alter the tumor immune microenvironment and enhance tumor immune escape.

关 键 词：TGF-Β1 EGFR突变 细胞因子 非小细胞肺癌 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]