SCG5在舌鳞状细胞癌中的表达及作用  

Expression and function of SCG5 in tongue squamous cell carcinoma

作　　者：郝斌 赵安邦 陈雄 HAO Bin;ZHAO Anbang;CHEN Xiong(Department of Otolaryngology,Head and Neck Surgery,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院耳鼻咽喉头颈外科,武汉430071

出　　处：《数理医药学杂志》2025年第4期258-266,共9页Journal of Mathematical Medicine

摘　　要：目的探究SCG5在舌鳞状细胞癌(tongue squamous cell carcinoma,TSCC)中的表达情况及其对TSCC细胞增殖、侵袭和迁移能力的影响。方法通过公共数据库评估SCG5在临床患者中的表达情况及其与预后的相关性;构建SCG5稳定敲低和过表达的TSCC细胞模型,采用细胞计数试剂盒-8(cell counting kit-8,CCK-8)、平板克隆、Transwell和划痕实验评估TSCC细胞的增殖、侵袭和迁移能力。利用实时荧光定量聚合酶链反应、Western blot检测TSCC细胞系中SCG5及上皮间充质转化(epithelial-mesenchymal transition,EMT)相关蛋白的表达情况。利用基因富集分析筛选SCG5的靶基因CYP450,采用基因相关性分析进一步验证两者之间的关系,随后通过Western blot检测SCG5下调后CYP450家族相关基因的表达情况。结果SCG5在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)组织中高表达,且与不良临床预后显著相关。下调SCG5表达抑制了TSCC细胞的增殖、侵袭和迁移能力及EMT过程;相反,过表达SCG5则增强了TSCC细胞的增殖和侵袭能力。基因富集分析结果显示,SCG5与药物代谢CYP450通路显著相关;基因相关性分析表明,CYP4F3与SCG5表达呈负相关。Western blot检测发现CYP450家族相关基因在SCG5基因敲低后表达增加。结论SCG5在HNSCC组织及TSCC细胞中高表达,下调SCG5可显著抑制TSCC细胞的增殖、侵袭、迁移和EMT过程,而SCG5过表达则会增强这些恶性行为。SCG5与CYP450的互作关系可能在TSCC的发展中发挥重要作用。Objective To investigate the expression of SCG5 in tongue squamous cell carcinoma(TSCC)and its impact on the proliferation,invasion,and migration abilities of TSCC cells.Methods The expression of SCG5 in clinical patient samples and its correlation with prognosis were assessed through public databases.Stable SCG5 knockdown and overexpressed TSCC cell models were constructed.Cell counting kit-8(CCK-8),colony formation,Transwell,and wound healing assays were used to evaluate the proliferation,invasion,and migration abilities of TSCC cells.Real-time quantitative polymerase chain reaction and Western blot were employed to measure the expression of SCG5 and epithelial-mesenchymal transition(EMT)-related proteins in TSCC cell lines.Gene enrichment analysis was conducted to screen for the target gene CYP450 of SCG5,and gene correlation analysis was performed to further validate their relationship.Subsequently,the expression of CYP450 family genes after SCG5 knockdown was examined by Western blot.Results SCG5 was highly expressed in head and neck squamous cell carcinoma(HNSCC)tissues and was significantly associated with the poor clinical prognosis.The knockdown of SCG5 expression inhibited the proliferation,invasion,migration,and EMT process of TSCC cells.In contrast,the overexpression of SCG5 enhanced the proliferation and invasion abilities of TSCC cells.Gene enrichment analysis revealed that SCG5 was significantly associated with the CYP450 drug metabolism pathway.Gene correlation analysis showed a negative correlation between CYP4F3 and SCG5 expression.Western blot results demonstrated that CYP450 family genes were upregulated after SCG5 knockdown.Conclusion SCG5 is highly expressed in HNSCC tissues and TSCC cells.The downregulation of SCG5 can significantly inhibits the TSCC cell proliferation,invasion,migration,and the EMT process,while the overexpression of SCG5 enhances these malignant behaviors.The interaction between SCG5 and CYP450 may play an important role in the development of TSCC.

关 键 词：SCG5 CYP450 舌鳞状细胞癌 上皮间充质转化 

分 类 号：R739.86[医药卫生—肿瘤]