UTP14a与c-myc在乳腺癌组织中的表达与临床意义  

Expression and clinical significance of UTP14a and c-myc in breast cancer tissues

作　　者：孟祥宇 张茂 刘鹏程 金志成 唐奇 MENG Xiangyu;ZHANG Mao;LIU Pengcheng;JIN Zhicheng;TANG Qi(Baotou Medical College,Inner Mongolia University of Science&Technology,Baotou 014040,Inner Mongolia Autonomous Region,China;Department of Thyroid and Breast Surgery,Baotou City Central Hospital,Baotou 014040,Inner Mongolia Autonomous Region,China;Department of Head and Neck Surgery,Baotou Cancer Hospital,Baotou 014030,Inner Mongolia Autonomous Region,China;Department of Cardiothoracic Surgery,The Second Affiliated Hospital of Baotou Medical College,Baotou 014040,Inner Mongolia Autonomous Region,China)

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014040 [2]包头市中心医院甲乳外科,内蒙古包头014040 [3]包头市肿瘤医院头颈外科,内蒙古包头014030 [4]包头医学院第二附属医院心胸外科,内蒙古包头014040

出　　处：《数理医药学杂志》2025年第4期267-275,共9页Journal of Mathematical Medicine

摘　　要：目的通过生物信息学分析及免疫组化实验探讨c-myc和UTP14a在乳腺癌组织及癌旁组织的差异表达、诊断和预后价值。方法从癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库获取癌症患者的UTP14a、c-myc基因表达和临床信息数据,并进行生存表达分析。对92例乳腺癌患者癌组织样本进行UTP14a、c-myc的免疫组化染色,采用χ^(2)检验和Spearman相关系数分析UTP14a与c-myc在不同亚组的表达水平与预后的关系。结果受试者工作特征(receiver operating characteristic,ROC)曲线分析结果显示,UTP14a在1年、3年和5年的ROC曲线下面积(area under curve,AUC)分别为0.559(95%CI:0.445~0.672)、0.542(95%CI:0.480~0.605)和0.545(95%CI:0.488~0.603);KM生存曲线分析结果显示,UTP14a高风险组和低风险组之间的生存差异无统计学意义(P=0.083)。c-myc在1年、3年和5年的AUC值分别为0.629(95%CI:0.550~0.708)、0.530(95%CI:0.460~0.600)和0.514(95%CI:0.438~0.590);KM生存曲线分析结果显示,c-myc高风险组和低风险组之间的生存差异无统计学意义(P=0.149)。免疫组化实验结果显示,UTP14a与c-myc主要在癌细胞的胞核和胞浆表达,肿瘤组织内UTP14a、c-myc阳性表达率高于正常组织(P<0.05);UTP14a在乳腺癌组织中的表达与患者年龄、组织学分级以及腋窝淋巴结转移率显著相关(P<0.05);c-myc在乳腺癌组织中的表达与患者组织学分级、腋窝淋巴结转移率、神经侵犯比例及雌激素受体(estrogen receptor,ER)表达阳性显著相关(P<0.05)。c-myc抗体和UTP14a抗体表达之间存在正相关性(P<0.05)。结论UTP14a和c-myc的联合检测可能为乳腺癌患者的治疗提供重要参考,帮助临床医生更好地进行个体化治疗。未来有待在更大规模的队列中验证本研究结果,以支持其在临床实践中的应用。Objective To explore the differential expression,diagnostic and prognostic value of c-myc and UTP14a in breast cancer tissues and adjacent tissues through bioinformatics analysis and immunohistochemical experiments.Methods UTP14a and c-myc gene expression and clinical information data of cancer patients were obtained from The Cancer Genome Atlas(TCGA)database,and survival expression was analyzed.Immunohistochemical staining of UTP14a and c-myc was performed on cancer tissue samples from 92 breast cancer patients.The relationship between the expression levels of UTP14a and c-myc in different subgroups and prognosis was analyzed usingχ^(2) test and Spearman correlation coefficient.Results The results of receiver operating characteristic(ROC)curve analysis showed that the area under curve(AUC)values of UTP14a at 1 year,3 and 5 years were 0.559(95%CI:0.445-0.672),0.542(95%CI:0.480-0.605)and 0.545(95%CI:0.488-0.603),respectively.The results of KM survival curve analysis showed that there was no significant difference in survival between UTP14a high risk and low risk groups(P=0.083).The AUC values of c-myc at 1 year,3 and 5 years were 0.629(95%CI:0.550-0.708),0.530(95%CI:0.460-0.600)and 0.514(95%CI:0.438-0.590),respectively.The results of KM survival curve analysis showed that there was no significant difference in survival between c-myc high risk and low risk groups(P=0.149).The results of immunohistochemical experiments showed that UTP14a and c-myc were mainly expressed in the nucleus and cytoplasm of cancer cells,and the positive expression rates of UTP14a and c-myc in tumor tissues were higher than those in normal tissues(P<0.05).The expression of UTP14a in breast cancer tissues was significantly correlated with the patient's age,histological grading,and the rate of metastasis to axillary lymph nodes(P<0.05).The expression of c-myc in breast cancer tissues was significantly correlated with histological grading,the rate of metastasis to axillary lymph nodes,nerve invasion ratio and positive expression of estrogen r

关 键 词：UTP14a C-MYC 乳腺癌 生物信息学 免疫组化 

分 类 号：R737.9[医药卫生—肿瘤]