免疫磁珠分离技术在食品诺如病毒检测中的应用前景  

The application prospects of immunomagnetic bead separation technology in the detection of food norovirus

作　　者：刘萍 郦娟 向春燕 高家昊 付诗慧 晏涛 曾慧君 LIU Ping;LI Juan;XIANG Chunyan;GAO Jiahao;FU Shihui;YAN Tao;ZENG Huijun(Wuhan Institute for Food and Cosmetic control,Hubei Wuhan 430012,China)

机构地区：[1]武汉食品化妆品检验所,湖北武汉430012

出　　处：《食品工程》2025年第1期1-4,10,共5页Food Engineering

基　　金：国家市场监督管理总局科技计划项目(2020MK131);湖北省市场监督管理局技术保障和科技计划项目(Hbscjg-JS2021007)。

摘　　要：诺如病毒(Norovirus,NoV)是引起食源性疾病的主要病原体。由于食品中诺如病毒浓度较低,在实际检测中,病毒的分离富集一直是食品中诺如病毒检测中的难点。免疫磁珠可以通过偶联特异性抗体,利用外加磁场分离富集复杂基质中的诺如病毒。该方法操作步骤简单、用时短、对人员的技术要求较低,并可以对抗原进行特异性识别,排除其他杂质的干扰,避免食品中的复杂基质对后续检测的影响,为食品基质中低拷贝诺如病毒检测提供了一个有效的富集方法。主要综述了免疫磁珠分离技术(immunomagnetic separation,IMS)及其可能的影响因素、在食品诺如病毒检测中的应用优势、与其他检测技术联用的前景等,以期为免疫磁珠分离技术在诺如病毒快速检测中的综合应用提供参考。Norovirus is the main pathogen causing foodborne diseases worldwide.Due to the low concentration of norovirus in food,virus isolation and enrichment have always been a challenge in actual detection of norovirus in food.Immunomagnetic beads can separate and enrich norovirus in complex matrices by coupling specific antibodies and utilizing an external magnetic field.This method has simple operation steps,shorter duration,and lower technical requirements for personnel.It can specifically identify the antigen,and eliminate the interference of other impurities.It can also avoid the influence of complex matrices in food on subsequent detection.This provides an effective enrichment method for detecting low copy norovirus in food matrices.This article mainly reviews the immunomagnetic bead separation technology and its possible influencing factors,the advantages of its application in detecting norovirus in food,and the prospects of its combination with other detection technologies,in order to provide reference for the comprehensive application of immunomagnetic bead separation technology in rapid detection of norovirus.

关 键 词：免疫磁珠分离 诺如病毒检测 应用前景 

分 类 号：TS207.4[轻工技术与工程—食品科学]