UPLC-MS分析五指毛桃配制酒中3种线型呋喃香豆素物质的含量  

Analysis of the content of three linear furanocoumarin substances in Ficus hirta Vahl.liquor by ultra-high performance liquid chromatography-mass spectrometry

作　　者：陈子凡 池少铃 伍莉婷 黄乐喜 CHEN Zifan;CHI Shaoling;WU Liting;HUANG Lexi(Heyuan Food Inspection Institute,Guangdong Heyuan 517000,China;HeyuanDrug Inspection Institute,Guangdong Heyuan 517000,China;Heyuan Yuancheng District agricultural products quality supervision,inspection and testing station,Guangdong Heyuan 517000,China;Heyuan Nanqi Agricultural Development Co.,Ltd.,Guangdong Heyuan 517000,China)

机构地区：[1]河源市食品检验所,广东河源517000 [2]河源市药品检验所,广东河源517000 [3]河源市源城区农产品质量监督检验检测站,广东河源517000 [4]河源市南芪农业发展有限公司,广东河源517000

出　　处：《食品工程》2025年第1期71-75,共5页Food Engineering

基　　金：河源市科技计划项目(230613091602307)。

摘　　要：建立了一种利用超高效液相色谱-质谱仪联用法(ultra high performance liquid chromatography-mass spectrometry,UPLC-MS)分析五指毛桃配制酒中补骨脂素、8-甲氧基补骨脂素和佛手苷内酯3种线型呋喃香豆素的方法。采用微量注射泵直接进样,使用Tune软件在ESI+模式下测得3种物质的前体离子和产物离子,建立SRM定量检测方法。结果表明,3种物质的检测方法线性良好,在2.5~250 ng/mL质量浓度范围内相关系数均在0.995以上,回收率为89.6%~112.4%,变异系数为1.36%~2.99%,检出限为0.5~2.5μg/kg,定量限为2~5μg/kg。本方法作为五指毛桃配制酒中3种线型呋喃香豆素定性和定量测定方法,具有简单快捷、效率高、定量准确、灵敏度高、重复性好的特点。This article established a method for the analysis of three linear furanocoumarins,psoralen,8-methoxypsoralen,and bergapten,in Ficus hirta Vahl.mixed liquor by using ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS).Microinjection pumps were used for direct injection,and the precursor ions and product ions of the three substances were measured using Tune software in ESI+mode to establish an SRM quantitative detection method.The results showed that the detection method for the three substances had good linearity.While the content was between 2.5 and 250 ng/mL,the correlation coefficients were above 0.995.The recovery rate was between 89.6% and 112.4%,and the CV was between1.36% and 2.99%.The detection limit was between 0.5 and 2.5μg/kg,and quantitative limit was between2 and 5μg/kg.This method,as a qualitative and quantitative determination method for the three linear furanocoumarins in Ficus hirta Vahl.mixed liquor,is characterized by simplicity,rapidity,high efficiency,accurate quantitation,high sensitivity,and good repeatability.

关 键 词：超高效液相色谱-质谱联用法(UPCC-MS) 五指毛桃 补骨脂素 8-甲氧基补骨脂素 佛手苷内酯 

分 类 号：TS207.51[轻工技术与工程—食品科学]