CCL7在非小细胞肺癌中的表达及与免疫浸润和预后的相关性分析  

Analysis of CCL7 expression in non-small cell lung cancer and its correlation with immune infiltration and patient prognosis

作　　者：孟祥淑 尤德胜 王瑞瑞 王亚举 马彩云 王春景 MENG Xiang-shu(School of Life Sciences,Bengbu Medical University,Bengbu 233000,Anhui,China)

机构地区：[1]蚌埠医科大学生命科学学院,安徽蚌埠233000

出　　处：《牡丹江医科大学学报》2025年第2期19-25,60,共8页Journal of Mudanjiang Medical University

基　　金：安徽省高等学校自然科学研究重点项目(2024AH051296);安徽省质量工程“生物科学拔尖学生培养基地”项目(2021jcxkpy014);安徽省质量工程教学研究重点项目(2020jyxm1176);国家级大学生创新创业训练计划项目(202410367002)。

摘　　要：目的探讨CC趋化因子配体7(C-C Chemokine Ligand 7,CCL7)在非小细胞肺癌(Non-Small-Cell Lung Carcinoma,NSCLC)中的表达情况,并分析CCL7与NSCLC免疫浸润及预后的关系。方法利用生物信息学方法,通过UALCAN数据库和TIMER数据库分析CCL7在NSCLC及不同肿瘤中的表达。通过LogPc数据库分析CCL7表达对NSCLC临床预后的影响。采用TIMER数据库分析CCL7表达与NSCLC免疫浸润的相关性。利用GEPIA数据库分析CCL7表达与肿瘤浸润免疫细胞(Tumor Infiltrating Immune Cells,TIICs)表面标志物的相关性。采用Kaplan-Meier Plotter数据库分析基于免疫细胞亚群的CCL7表达与NSCLC患者预后的相关性。结果与癌旁正常组织相比,CCL7 mRNA在NSCLC癌组织表达显著升高(P<0.05)。CCL7表达与NSCLC的组织病理类型、临床分期和淋巴结转移有关(P<0.05)。CCL7 mRNA高表达与NSCLC患者较差的预后相关(P<0.05)。CCL7 mRNA表达与NSCLC中TIICs水平呈正相关(P<0.05),其中包括B细胞、CD8^(+)T细胞、CD4^(+)T细胞、巨噬细胞、中性粒细胞和树突状细胞。CCL7 mRNA水平与NSCLC中B细胞、CD8^(+)T细胞、CD4^(+)T细胞、巨噬细胞等16种TIICs的表面标志物水平呈正相关(P<0.05)。免疫浸润可作为CCL7高表达导致LUAD和LUSC患者不良预后的因素(P<0.05)。结论CCL7在NSCLC癌组织中高表达并上调NSCLC免疫浸润水平,进而导致NSCLC不良预后的发生。因此,CCL7有望作为NSCLC免疫浸润相关的预后生物标志物。Objective To investigate CCL7 expression in non-small cell lung cancer(NSCLC)and analyze its association with immune infiltration and prognosis.Methods CCL7 expression in NSCLC and other tumors was analyzed using the UALCAN and TIMER databases.The impact of CCL7 expression on NSCLC prognosis was assessed through the LogPc database,and its correlation with immune infiltration was explored via TIMER.The GEPIA database was used to analyze the relationship between CCL7 and tumor-infiltrating immune cells(TIICs)markers,while Kaplan-Meier Plotter evaluated the correlation of CCL7 expression and prognosis of NSCLC patients based on immune cell subsets.Results CCL7 mRNA expression was significantly higher in NSCLC tumor tissues than in normal tissues(P<0.05).CCL7 expression in NSCLC was associated with the histopathological type,clinical stage and lymph node metastasis of NSCLC(P<0.05).High CCL7 expression correlated with poor prognosis in NSCLC(P<0.05).CCL7 mRNA was positively correlated with the surface markers of 16 TIICs in NSCLC,such as B cells,CD8^(+)T cells,CD4^(+)T cells and macrophages.Immune infiltration may be a factor contributing to the poor prognosis of NSCLC patients with high CCL7 expression(P<0.05).Conclusion CCL7 is highly expressed in NSCLC tumor tissues and upregulates the level of NSCLC immune infiltration,which leads to the occurrence of poor prognosis of NSCLC.Therefore,CCL7 can serve as a prognostic biomarker for NSCLC immune infiltration.

关 键 词：CC趋化因子配体7 非小细胞肺癌 免疫浸润 肿瘤微环境 

分 类 号：R734.2[医药卫生—肿瘤]