一株PHB降解菌株的分离及其PHB降解特性研究  

Isolation of a PHB Degrading Bacterium Strain and Characterization of PHB Degradation Conditions

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作  者:王玉蝶 高美荣 马颖超 隋丽英 WANG Yudie;GAO Meirong;MA Yingchao;SUI Liying(College of Marine and Environmental Sciences,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区:[1]天津科技大学海洋与环境学院,天津300457

出  处:《天津科技大学学报》2025年第2期37-43,共7页Journal of Tianjin University of Science & Technology

摘  要:从海水鱼循环水养殖系统中分离筛选出一株降解聚-3-羟基丁酸酯(PHB)的菌株W7,结合其形态学、生理生化特性、16S rDNA序列比对和系统发育树分析进行物种鉴定,确定菌株W7为深海弯曲菌属(Thalassolituus)。将菌株W7接种于以PHB为唯一碳源的液体培养基中,研究培养基不同初始pH、盐度和温度对其降解PHB能力的影响。结果表明:菌株W7为革兰氏阴性菌,无鞭毛,无色透明,呈弯曲状,少量呈短棒状,能利用β-羟基-D,L-丁酸和L-乳酸等碳源,在盐度10~50、pH 5~8、28℃条件下能生长。菌株W7与食碱深海弯曲菌Thalassolituus alkanivorans TMPB967(OK489464)同源性为98.75%,为具有PHB降解能力的深海弯曲菌属菌株。菌株在培养1 d后,培养液pH均降至5.5左右;除盐度50组外,PHB解聚酶相对酶活力均在培养3 d时达到峰值;在28℃、pH 7.2和盐度30条件下培养5 d,PHB降解率最高达到(79.70±0.52)%。本研究获得了一株高效降解PHB的Thalassolituus W7并研究了菌株的PHB降解特性,为该菌株应用于海水环境中PHB的生物降解提供依据。In our present study,a poly-3-hydroxybutyrate(PHB)-degrading bacteria strain W7 was screened from a marine fish recirculating aquaculture system.The taxonomy identification was then performed by combining its morphology,physiological and biochemical characteristics,as well as 16S rDNA sequence alignment and phylogenetic tree analysis,and strain W7 was identified as Thalassolituus.The effects of initial pH and salinity of culture medium and incubation temperature on PHB degradation rate were finally investigated by inoculating strain W7 into liquid medium containing PHB as a sole carbon source.The results showed that strain W7 was identified as Gram-negative bacteria,with colorless and transparent colony and curved or rods-shaped cell without flagella.Strain W7 could utilize carbon sources such as β-hydroxy-D,Lbutyric acid and L-lactic acid,and grow in salinity range of 10-50,28℃ and pH 5-8.Strain W7 belonged to genus Thalassolituus which exhibited 98.75% similarity with T.alkanivorans TMPB967(OK489464).This is the first documented Thalassolituus strain within this genus that exhibits the capacity of PHB degradation.The pH of medium decreased to approximately 5.5 after one day incubation,while the relative PHB depolymerase activity reached its peak at day 3 except for that at salinity 50 which peaked at day 4.The highest PHB degradation rate was achieved after 5-day incubation at 28℃,pH 7.2 and salinity 30,reaching(79.70±0.52)%.In summary,our study identified strain W7 belonging to Thalassolituus with high PHB degradation capacity,which supports the applications of this strain in PHB biodegradation in marine environments.

关 键 词:聚-3-羟基丁酸酯(PHB) 生物降解 深海弯曲菌属 PHB降解率 PHB解聚酶相对酶活力 

分 类 号:Q939.9[生物学—微生物学] X172[环境科学与工程—环境科学]

 

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