火焰原子吸收光谱法检测蜂巢中8种金属元素含量及分布特征  

Content and distribution characteristics of 8 kinds of metal elements in honeycomb by flame atomic absorption spectrometry

作　　者：宋德云 范忠秧 黄颖 何爱明 谢自彤 刘号然 姚莎 张孟扬 陈红云 SONG De-Yun;FAN Zhong-Yang;HUANG Ying;HE Ai-Ming;XIE Zi-Tong;LIU Hao-Ran;YAO Sha;ZHANG Meng-Yang;CHEN Hong-Yun(College of Public Health,Dali University,Dali 671000,China)

机构地区：[1]大理大学公共卫生学院,大理671000

出　　处：《食品安全质量检测学报》2025年第9期223-229,共7页Journal of Food Safety and Quality

基　　金：云南省昆虫生物医药研发重点实验室开放课题研究项目(AG2024010)。

摘　　要：目的 探究蜂巢中金属元素的分布规律及潜在食品安全风险。方法 本研究采用火焰原子吸收光谱法测定云南、广东、广西等地采集的蜂巢样品7个部位(巢膜、巢柱、巢壁、全巢、成蜂、巢底、蛹)中Cd、Fe、Cr、Mg、Zn、Cu、Mn和Pb等8种金属元素含量。结果 各元素标准曲线线性良好(相关系数r^(2)≥0.993),方法灵敏度满足痕量检测需求(检出限0.002~0.010 mg/kg)。蜂巢不同部位元素含量差异显著(P<0.05),平均含量排序为Mg (0.97 mg/kg)>Zn (0.15 mg/kg)>Mn (0.051 mg/kg)>Cu (0.0385 mg/kg)>Fe (0.02 mg/kg)>Cd (0.0060 mg/kg)>Pb (0.00027 mg/kg)>Cr (0.00003 mg/kg)。其中,Cd、Fe、Mg、Zn、Cu、Mn在部位间分布差异显著(P<0.05),而Cr、Pb无显著差异(P>0.05)。研究表明,必需元素(如Mg、Zn)的分布与蜂巢功能密切相关,而Cd、Pb的局部富集可能受环境输入影响。结论 本研究系统揭示了蜂巢金属元素的分布特征,为蜂产品质量控制及污染溯源提供了科学依据。Objective To explore the distribution of metal elements in honeycomb and the potential food safety risks.Methods In this study,flame atomic absorption spectrometry was used to determine the content of Cd,Fe,Cr,Mg,Zn,Cu,Mn and Pb in 7 parts(nest membrane,nest column,nest wall,whole nest,adult bee,nest bottom,pupae)of honeycomb samples collected from Yunnan,Guangdong and Guangxi.Results The standard curves exhibited excellent linearity with correlation coefficients(r~2)≥0.993,and the method sensitivity met trace detection requirements(limits of detection:0.002–0.010 mg/kg).Significant differences in metal content was observed among different parts of the honeycombs(P<0.05),with the average concentrations ranked as Mg(0.97 mg/kg)>Zn(0.15 mg/kg)>Mn(0.051 mg/kg)>Cu(0.0385 mg/kg)>Fe(0.02 mg/kg) >Cd(0.0060 mg/kg)>Pb(0.00027 mg/kg)>Cr(0.00003 mg/kg).The distributions of Cd,Fe,Mg,Zn,Cu and Mn showed significant variations across parts(P<0.05),whereas Cr and Pb exhibited no significant differences(P>0.05).The results suggestted that the distribution of essential elements(Mg and Zn) was closely associated with the biological functions of honeycombs,while the localized accumulation of Cd and Pb might reflect environmental contamination.Conclusion This study systematically reveals the spatial distribution characteristics of metal elements in honeycombs,providing a scientific foundation for quality control of bee products and traceability of environmental pollutants.

关 键 词：火焰原子吸收光谱法 蜂巢 金属元素分布 必需元素 重金属污染 食品安全评价 环境溯源 

分 类 号：O657.3[理学—分析化学]