4种植物次生物质对黄粉虫解毒酶活性及羧酸酯酶基因表达的影响  

Effects of four plant secondary metabolites on the detoxification enzyme activity and expression of carboxylesterase genes of Tenebrio molitor

作　　者：汪彤 李哲 张爽[1,2] WANG Tong;LI Zhe;ZHANG Shuang(College of Forestry,Hebei Agricultural University,Baoding 071000,China;Hebei Urban Forest Health Technology Innovation Center,Baoding 071000,China)

机构地区：[1]河北农业大学林学院,河北保定071000 [2]河北省城市森林健康技术创新中心,河北保定071000

出　　处：《江苏农业学报》2025年第3期501-509,共9页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31000305);河北省高等学校科学技术研究项目(QN2015182)。

摘　　要：为了探索京尼平、芸香苷、槲皮素和绿原酸对黄粉虫的作用机制,本研究通过测定黄粉虫取食4种植物次生物质后羧酸酯酶、细胞色素P450酶和谷胱甘肽S-转移酶活性,并用半定量PCR、荧光定量PCR法检测4种植物次生物质对黄粉虫羧酸酯酶基因(TmCarE)表达的诱导情况。结果表明,4种植物次生物质对黄粉虫3种解毒酶活性的诱导存在剂量效应和时间效应,部分低含量(0.5%、1.0%)的植物次生物质可以诱导并激活黄粉虫解毒酶活性,部分高含量(3.0%)的植物次生物质能显著抑制解毒酶活性。TmCarE具有羧酸酯酶基因的保守结构域,与已报道的昆虫羧酸酯酶基因在活性位点附近有较高的相似性。此外,含量为0.5%的3种植物次生物质(芸香苷、槲皮素和绿原酸)均可诱导TmCarE基因的相对表达量提高。本研究结果可为筛选高效的植物次生物质、科学利用植物次生物质进行鞘翅目害虫的防治提供理论依据。To explore the mechanisms of genipin,rutin,quercetin,and chlorogenic acid on Tenebrio molitor,the activities of carboxylesterase(CarE),cytochrome P450(CYP450),and glutathione S-transferase(GST)were measured after T.molitor fed on four plant secondary metabolites.Additionally,the expression of the carboxylesterase gene in T.molitor(TmCarE)was detected using semi-quantitative PCR and reverse transcription quantitative PCR(RT-qPCR)methods.The results showed that the induction of the three detoxification enzyme activities in T.molitor by plant secondary metabolites exhibited both dose-and time-dependent effects.Low contents(0.5%and 1.0%)of plant secondary metabolites could induce and activate the detoxification enzyme activities in T.molitor,while the high concent(3.0%)significantly inhibited these activities.Bioinformatics analysis revealed that TmCarE possessed conserved domains of the carboxylesterase gene and exhibited high similarity with other insect carboxylesterase genes near the active site.Moreover,three plant secondary metabolites(rutin,quercetin,and chlorogenic acid)at a content of 0.5%could up-regulate the relative expression of the TmCarE gene.These findings provide a theoretical basis for screening efficient plant secondary metabolites and scientifically utilizing them to control Coleoptera pests.

关 键 词：黄粉虫 植物次生物质 解毒酶 羧酸酯酶基因 

分 类 号：S763.3[农业科学—森林保护学]