厚壳贻贝外套膜对细菌肽聚糖胁迫的代谢响应  

METABOLIC RESPONSE OF MYTILUS CORUSCUS MANTLE TO PEPTIDOGLYCAN STRESS

作　　者：杨子霖 范孝俊 肖文慧 曹萍麟 张晓林 严小军 廖智 YANG Zi-Lin;FAN Xiao-Jun;XIAO Wen-Hui;CAO Ping-Lin;ZHANG Xiao-Lin;YAN Xiao-Jun;LIAO Zhi(Laboratory of Marine Biology Resource and Molecular Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,China)

机构地区：[1]浙江海洋大学海洋科学与技术学院海洋生物资源与分子工程实验室,舟山316022

出　　处：《水生生物学报》2025年第5期185-196,共12页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金面上项目(32271580);国家自然科学基金重点国际合作与交流项目(42020104009)资助。

摘　　要：为了解贻贝(Mytilus)外套膜免疫相关机制,对贻贝进行肽聚糖(Peptidoglycan,PGN)胁迫,并利用超高压液相色谱-质谱联用技术,对贻贝外套膜在PGN胁迫后48h的代谢物组成及含量进行组学鉴定,同时对胁迫前后的外套膜组织开展了游离氨基酸组成,外套膜黏液抑菌活性及抗氧化能力分析。结果表明,PGN胁迫导致贻贝外套膜部分代谢物含量发生显著变化(P<0.05),从中共鉴定到486种差异代谢物,包括232种上调和254种下调代谢物;其中,上调差异代谢物主要富集于细胞信号转导及氨基酸代谢相关途径,而下调SDM主要富集到脂质代谢和维生素代谢相关途径。此外,PGN胁迫导致外套膜中过氧化氢酶活力上调(P<0.05),并导致外套膜黏液对枯草芽孢杆菌的抑制率显著上升(P<0.05)。研究为深入了解贻贝应对免疫胁迫的分子策略,以及贻贝健康养殖奠定了基础。Mytilus coruscus is a shellfish with huge economic importance in China,and the mantle is an important immune-related organ.To investigate the immune function of the mantle and its underlying mechanisms,peptidoglycan(PGN)was used as a stressor,and ultra-high-pressure liquid chromatography-mass spectrometry technique was employed to identify the composition and content of metabolites from the mantle at 48h of post peptidoglycan stress.In addition,free amino acids,antimicrobial activities,and antioxidant activities of the mantle were analyzed and compared between the control and stressed samples.The results revealed that PGN stress induced significant changes in mantle metabolites,and a total of 486 metabolites with significant difference(SDMs)was identified,including 232 up-regulated and 254 down-regulated SDMs(P<0.05).Among these,lipids and lipid-like molecules,organic acids and their derivatives,and organo-heterocyclic compounds were the most prominent SDMs.KEGG enrichment analysis further revealed that these SDMs were enriched in different pathways,showing a complex response of the mantle to the PGN stress.Notably,the up-regulated SDMs were enriched in pathways related to cell signaling and amino acid metabolism,while down-regulated SDMs were enriched in lipid metabolisms,vitamin related metabolisms,and autophagy.Gene set enrichment analysis(GSEA)further confirmed the regulation of some SDMs and the enrichment of aminoacyl-tRNA biosynthesis and autophagy-animal.In addition,free amino acid analysis also confirmed the up-regulation(P<0.05)of certain amino acids,such as aspartic acid,glutamic acid,and arginine,in the mantle under PGN stress.Enzymatic activity analysis revealed an increase(P<0.05)of catalase activity and a decrease of hydrogen peroxide in the PGN stressed mussel mantle.Interestingly,antimicrobial function analysis revealed that the mucus from PGN stressed mantle presented stronger inhibition against Bacillus subtilis,indicating that PGN stress induce stronger antibacterial activity in mussel mantle.

关 键 词：外套膜 肽聚糖 免疫 代谢组学 厚壳贻贝 

分 类 号：Q789[生物学—分子生物学]