转运蛋白在细菌吸附、解毒重金属中的功能预测与分析  

Function prediction and analysis of transporters in adsorption and detoxification of heavy metals by bacteria

作　　者：孙尚琛 王永刚[2] 何碧红 陈臻 罗文[2] SUN Shangchen;WANG Yonggang;HE Bihong;CHEN Zhen;LUO Wen(Yellow River Basin Ecotope Integration of Industry and Education Research Institute,Lanzhou Resources&Environment Voc-Tech University,Lanzhou Gansu 730030;School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou Gansu 730050)

机构地区：[1]兰州资源环境职业技术大学黄河流域生态环境产教融合研究院,甘肃兰州730030 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《环境污染与防治》2025年第4期48-53,I0003,共7页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.32160066);甘肃省教育厅青年博士支持项目(No.2023QB-021);兰州资源环境职业技术大学校级重大科研项目(No.X2023ZD-01);甘肃省自然科学基金资助项目(No.24JRRA741)。

摘　　要：为研究转运蛋白在细菌吸附、解毒重金属中的作用,以一株从重金属污染土壤中分离出的细菌为研究对象,分别检测了菌株细胞组分对40~160 mg/L的Cu^(2+)、Zn^(2+)、Cd^(2+)和Cr(Ⅵ)的吸附能力,并分析了菌株基因组中与重金属吸附、解毒相关的转运蛋白。结果表明,菌株对120 mg/L的Cd^(2+)的吸附能力最强,吸附率为93.4%。对4种重金属的吸附方式主要是表面吸附,其次对Cu^(2+)和Zn^(2+)为胞外沉淀,对Cd^(2+)和Cr(Ⅵ)为胞内积累。在菌株基因组中,ABC转运蛋白家族的蛋白质参与重金属的转运和吸附,包括磷酸盐转运家族PstSPstBCA、MlaF和MlaE等。NRAMP家族中的MntH蛋白、ZIP家族中的ZupT蛋白因结构中存在大量金属离子结合位点,可为菌株对重金属的转运和胞内积累提供载体。外排系统中的2个CDF家族蛋白、10个RND蛋白和5个P-ATPase蛋白因其特殊结构域通过对金属离子的外排实现菌株对重金属的解毒。To study the role of transporters in adsorption and detoxification of heavy metals by bacteria,the bacteria isolated from heavy metal contaminated soil was studied.The adsorption properties of 40-160 mg/L of Cu^(2+),Zn^(2+),Cd^(2+)and Cr(Ⅵ)were investigated,and the transporters related to the adsorption and detoxification of heavy metals were analyzed.The results showed that the adsorption capacity of 120 mg/L of Cd^(2+)was the strongest(adsorption rate was 93.4%).Furthermore,the four heavy metals were mainly adsorbed by surface adsorption,followed by extracellular precipitation for Cu^(2+)and Zn^(2+),while intracellular accumulation for Cd^(2+)and Cr(Ⅵ).In the strain genome,proteins belonging to ABC transporter family participated the transport and biosorption of heavy metals,including the phosphate transport family PstSPstBCA,MlaF and MlaE.The MntH protein in the NRAMP family and ZupT protein in the ZIP family could provide a carrier for the transport and intracellular accumulation of heavy metals due to the presence of a large number of metal ion binding sites in their structures.In addition,two CDF family proteins,ten RND proteins and five P-ATPase proteins were involved in the efflux of metal ions and detoxification by bacteria due to their special domains.

关 键 词：转运蛋白 细菌 重金属 吸附 解毒 功能 

分 类 号：X172[环境科学与工程—环境科学]