Metabolome and RNA-seq reveal discrepant metabolism and secretory metabolism profile in skeletal muscle between obese and lean pigs at different ages  

作　　者：Liu Guo Junfei Xu Wenyue Zhou Sisi Chen Hanjing Shi Mengmeng Han Zekun Yang Yehui Duan Weijun Pang Yulong Yin Fengna Li 

机构地区：[1]Laboratory of Animal Nutritional Physiology and Metabolic Process,Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,410125,China [2]University of Chinese Academy of Sciences,Beijing,101408,China [3]College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing,100049,China [4]Laboratory of Animal Fat Deposition and Muscle Development,College of Animal Science and Technology,Northwest A&F University,Yangling,712100,China

出　　处：《Science China(Life Sciences)》2025年第4期1102-1117,共16页中国科学(生命科学英文版)

基　　金：supported by the National Natural Science Foundation of China(31972582);the STI 2030-Major Project(2023ZD04072);the Major Project of Changsha City(kh2401015);the Science and Technology Innovation Program of Hunan Province(2021RC4039);the Key Research and Development Program of Hunan Province(2022NK2026);the Youth Innovation Promotion Association CAS(Y202079);China Agriculture Research System of MOF and MARA(CARS-35)。

摘　　要：Metabolites and metabolism-related gene expression profiles in skeletal muscle change dramatically under obesity,aging and metabolic disease.Since obese and lean pigs are ideal models for metabolic research.Here,we compared metabolome and transcriptome of Longissimus dorsi(LD)muscle between Taoyuan black(TB,obese)and Duroc(lean)pigs at different ages.We defined the“window phase”of intramuscular fat(IMF)deposition in TB pig,which has significantly higher IMF than Duroc pig.Our results displayed discrepant lipid composition and different expression genes(DEGs)enriched in lipid metabolism,and both metabolome and transcriptome analyses revealed stronger energy expenditure and more active amino acid and protein metabolism in Duroc pig.10 up-and 51 down-regulated biomarker metabolites with age-and breed-specificity were identified.Potential secretory metabolites,including organic acid(fumaric acid,succinate,malic acid,and gamma-aminobutyric acid),amino acid(L-lysine,and L-glutamic acid),lipid(2-hydroxyisovaleric acid,and L-carnitine)were demonstrated a significant correlation with IMF deposition.Our research highlights the huge difference of metabolic spectrum in skeletal muscle between obese and lean model and muscle-derived secretory metabolites might act as an ambassador of intercellular communication to regulate systematic metabolism.

关 键 词：skeletal muscle METABOLOME transcriptome biomarker metabolite intercellular communication lipid deposition pig model 

分 类 号：S828[农业科学—畜牧学]