单核细胞TLR4+896、+1196基因多态性与缺血性脑卒中患者并发泌尿系感染的关系  

The Relationship between monocyte TLR4+896 and +1196 gene polymorphism and urinary tract infection in patients with ischemic stroke

作　　者：朱喜悦 刘佳[1] 张洁[1] ZHU Xiyue;LIU Jia;ZHANG Jie(Department of Geriatrics,South Campus,Sixth People's Hospital Affiliated to Shanghai Jiao Tong University,Shanghai 201400,China)

机构地区：[1]上海交通大学附属第六人民医院南院老年医学科,上海201400

出　　处：《中南医学科学杂志》2025年第2期245-248,共4页Medical Science Journal of Central South China

基　　金：上海市“科技创新行动计划”医学创新研究领域项目(20210203)。

摘　　要：目的 探讨单核细胞Toll样受体4(TLR4)+896、+1196基因多态性与缺血性脑卒中(CIS)并发泌尿系感染的关系。方法 选取CIS并发泌尿系感染患者57例(泌尿感染组)和同期未并发泌尿系感染的CIS患者50例(对照组)。采用流式细胞术检测单核细胞表面TLR4表达水平,采用PCR结合测序检测TLR4位点+896A/G、+1196C/T的单核苷酸多态性。采用Logistic回归分析泌尿系感染的影响因素。结果 泌尿感染组TLR4水平以及导尿管置管、合并糖尿病患者比例高于对照组(P<0.05)。与对照组比较,泌尿感染组TLR4+896 AA基因型、A频率降低,TLR4+896 GG基因型、G频率和TLR4+1196 TT基因型、T频率升高(P<0.05)。Logistic回归分析显示,导尿管置管、合并糖尿病、TLR4+896G/A是CIS患者发生泌尿系感染的影响因素(P<0.05)。与无导尿管置管患者比较,导尿管置管患者TLR4+896基因A频率低,基因G频率高(P<0.05)。结论 导尿管置管、合并糖尿病是CIS患者并发泌尿系统感染的危险因素,TLR4+896G/A与CIS并发泌尿系感染关系密切。Aim To investigate the relationship between Toll-like receptor 4(TLR4) +896 and +1196 gene polymorphisms in monocytes and the occurrence of urinary tract infections in patients with cerebral ischemic stroke(CIS).Methods Fifty-seven CIS patients with UTIs(UTI group) and 50 CIS patients without UTIs(control group) were selected.Flow cytometry was used to detect TLR4 expression levels on monocyte surfaces,and PCR combined with sequencing was used to detect single nucleotide polymorphisms(SNPs) at TLR4 loci +896A/G and +1196C/T.Logistic regression analysis was performed to identify influencing factors of UTIs.Results The levels of TLR4,as well as the proportions of patients with indwelling catheters and comorbid diabetes,were significantly higher in the urinary infection group compared to the control group(P<0.05).Compared with the control group,the urinary infection group exhibited a lower frequency of the TLR4+896 AA genotype and allele A,while the frequencies of the TLR4+896 GG genotype,allele G,TLR4+1196 TT genotype,and allele T were significantly higher(P<0.05).Logistic regression analysis revealed that indwelling catheters,comorbid diabetes,and TLR4+896G/A were influencing factors for urinary tract infections in CIS patients(P<0.05).Compared with patients without indwelling catheters,those with indwelling catheters had a lower frequency of the TLR4+896 allele A and a higher frequency of allele G(P<0.05).Conclusion Catheterization and diabetes are risk factors for UTIs in CIS patients,and TLR4+896G/A is associated with the occurrence of UTIs in CIS patients.

关 键 词：TOLL样受体4 缺血性脑卒中 泌尿系感染 基因多态性 

分 类 号：R743[医药卫生—神经病学与精神病学]