丛生盔形珊瑚转录组微卫星标记的分离和鉴定  

Development of novel EST-SSR markers for the stony coral Galaxea fascicularis

作　　者：卢章旺 李福宇 廖婷馨 李立微 王嫣[1] LU Zhangwang;LI Fuyu;LIAO Tingxin;LI Liwei;WANG Yan(School of Marine Biology and Fisheries,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学海洋生物与水产学院,海南海口570228

出　　处：《海南大学学报(自然科学版中英文)》2025年第1期25-32,共8页Natural Science of Hainan University

基　　金：国家科技基础资源调查专项课题(2022FY100604);国家自然科学基金项目(41376174)。

摘　　要：丛生盔形珊瑚是中国南海岛礁造礁珊瑚的代表性物种。根据其刺丝囊形态与线粒体基因组CYTB-ND2基因间区插入/缺失(indel)的基因型,丛生盔形珊瑚可分为S和H两种形态型,且二者的遗传和生态表型有明显的分化。本研究通过挖掘丛生盔形珊瑚编码蛋白的转录本序列的微卫星资源,分离和鉴定了11个在两种形态型中均能稳定而均衡扩增的EST-SSR分子标记,并分别在一个S群体(n=16)和一个H群体(n=31)中进行检测和验证。这11个标记均呈现多态性,在S群体,其等位基因数为3~8个(平均4.8±1.9个),观测杂合度(H_(O))为0.06~0.92(0.55±0.26),期望杂合度(H_(E))为0.24~0.83(0.40±0.27);在H群体,其等位基因数为2~9个(4.5±2.1),观测杂合度(HO)为0.17~1.00(0.70±0.15),期望杂合度(HE)为0.46~0.86(0.62±0.19)。仅一个位点(Gf-E24)因存在无效等位基因,在H群体中偏离哈迪-温伯格平衡。Galaxea fascicularis is a representative species of reef-building coral in the South China Sea.Based on the nematocyst morphology and the indel genotype of CYTB-ND2 in mitochondrial genome,G.fascicularis can be divided into two morphotypes:S and H,and there is significant differentiation in the genetic and ecological phenotypes between these two morphotypes.By mining microsatellite resources from the transcriptome sequences,we developed 11 SSR markers that could be used in both morphotypes,and tested and validated them in one S population(n=16)and one H population(n=31),respectively.All the 11 markers showed polymorphism.In S population,the number of alleles ranged from 3 to 8(mean 4.8±1.9),the observed heterozygosity(H_(O))ranged from 0.06 to 0.92(0.55±0.26),and the expected heterozygosity(HE)ranged from 0.24 to 0.83(0.40±0.27).In H population,the number of alleles were 2~9(4.5±2.1),the observed heterozygosity(H_(O))was 0.17~1.00(0.70±0.15),and the expected heterozygosity(HE)was 0.46~0.86(0.62±0.19).Only one locus(Gf-E24)deviated from the Hardy-Weinberg equilibrium in Hpopulation,probably due to the presence of null allele.

关 键 词：丛生盔形珊瑚 EST-SSR 形态型 线粒体基因型 遗传分化 

分 类 号：S917.4[农业科学—水产科学]