小茴香不同部位醇提取物的抑菌活性研究  

Research on Antibacterial Activity of Alcohol Extracts from Different Parts of Foeniculum vulgare Mill

作　　者：张宇泽 杨晓倩 王绍江 欧家成 牛永 ZHANG Yu-ze;YANG Xiao-qian;WANG Shao-jiang;OU Jia-cheng;NIU Yong(School of Medicine,Lijiang Culture and Tourism College,Lijiang 674199,Yunnan)

机构地区：[1]丽江文化旅游学院医学院,云南丽江674199

出　　处：《抗感染药学》2025年第1期6-10,共5页Anti-infection Pharmacy

基　　金：丽江文化旅游学院校级科研项目(编号:2021XK04,2022XY21,2023XY02)。

摘　　要：目的:比较小茴香不同部位醇提取物的抑菌活性,为小茴香的进一步药用开发提供参考。方法:采用20%乙醇溶液提取小茴香根、枝叶、果实的有效成分,随后用滤纸片法和微量稀释法测定小茴香根、枝叶、果实的醇提取物对金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌的抑菌圈直径和最低抑菌浓度(minimal inhibitory concentration,MIC),比较小茴香不同部位醇提取物的抑菌活性。结果:滤纸片法检测结果显示,小茴香根、枝叶、果实的醇提取物对金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌均具有抑菌活性,其中小茴香果实的醇提取物对金黄色葡萄球菌、大肠埃希菌的抑菌活性最强,而小茴香根的醇提取物对枯草芽孢杆菌的抑菌活性最强;微量稀释法检测结果显示,小茴香根、枝叶、果实的醇提取物对金黄色葡萄球菌的MIC分别为5.00、10.00、5.00 mg/mL,对大肠埃希菌的MIC均为10.00 mg/mL,而对枯草芽孢杆菌的MIC分别为20.00、20.00、10.00 mg/mL。结论:小茴香根、枝叶、果实的醇提取物对金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌均具有抑菌活性,其中对金黄色葡萄球菌的抗菌作用最强,其次为大肠埃希菌,再次为枯草芽孢杆菌。Objective:To compare the antibacterial activity of alcohol extracts from different parts of Foeniculum vulgare Mill.,and provide reference for further medicinal development of Foeniculum vulgare Mill.Methods:Effective components from roots,branches and leaves,and fruits of Foeniculum vulgare Mill.were extracted using 20%ethanol solution.The inhibitory zone diameters and minimal inhibitory concentrations(MIC)of alcohol extracts from roots,branches and leaves,and fruits of Foeniculum vulgare Mill.against Staphylococcus aureus,Escherichia coli,and Bacillus subtilis were measured using the filter paper disc method and microdilution method,then the antibacterial activity of alcohol extracts from different parts of Foeniculum vulgare Mill.was compared.Results:The detection results with the filter paper disc method showed that alcohol extracts from roots,branches and leaves,and fruits of Foeniculum vulgare Mill.exhibited antibacterial activity against Staphylococcus aureus,Escherichia coli,and Bacillus subtilis.The alcohol extracts from fruits of Foeniculum vulgare Mill.demonstrated the strongest antibacterial activity against Staphylococcus aureus and Escherichia coli,while the alcohol extracts from roots of Foeniculum vulgare Mill.showed the strongest antibacterial activity against Bacillus subtilis.The detection results with the microdilution method indicated that MIC values of alcohol extracts from roots,branches and leaves,and fruits of Foeniculum vulgare Mill.against Staphylococcus aureus were 5.00,10.00,and 5.00 mg/mL respectively,MIC values against Escherichia coli were 10.00 mg/mL uniformly,and MIC values against Bacillus subtilis were 20.00,20.00,and 10.00 mg/mL respectively.Conclusion:Alcohol extracts from roots,branches and leaves,and fruits of Foeniculum vulgare Mill.exhibit strong antibacterial activity against Staphylococcus aureus,Escherichia coli,and Bacillus subtilis,with the highest antibacterial effect on Staphylococcus aureus,followed by Escherichia coli,and Bacillus subtilis.

关 键 词：小茴香 醇提取物 抑菌活性 金黄色葡萄球菌 大肠埃希菌 枯草芽孢杆菌 

分 类 号：R285[医药卫生—中药学]